Publication
Biochip with E. coli bacteria for detection of arsenic in drinking water
Publications associées (37)
Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine
In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...
EPFL2023Microfluidic devices
The present disclosure relates to a microfluidic device comprising: a substrate; a culture chamber (130); a loading channel (170) in fluid communication with the culture chamber; at least one auxiliar channel (170-1, 5 170-2) extending from and in fluid co ...
2023Studying the roundworm Caenorhabditis elegans using microfluidic chips
C. elegans is an attractive model organism in biology, as it shows genetic similarity with humans, facilitates microscopic observation due to its transparency, and has a short life cycle. Moreover, many mutants expressing fluorescent proteins in particular ...
SPIE-INT SOC OPTICAL ENGINEERING2019Integration of microfluidics and multimodal optics for translational bioanalytics
Numerous analytical techniques for life sciences have been at the basis of the improvement of medical diagnostics and treatments. Current challenges in bioanalytics are related to the conception of methods enabling large amount of information to be extract ...
EPFL2019Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips
Harald Van Lintel, Jan Roelof van der Meer, Clémence Roggo
Whole-cell bacterial bioreporters are proposed asalternatives to chemical analysis of, for example, pol-lutants in environmental compartments. Commonlybased on reporter gene induction, bioreporters pro-duce a detectable signal within 30 min to a few hoursa ...
Wiley-Blackwell2018A Microfluidic Biodisplay
Sebastian Maerkl, Francesca Volpetti, Ekaterina Emilova Petrova
Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves ...
American Chemical Society2017Microfluidic staining technology and automated image processing for fast and accurate tissue-based diagnostics
The development of microfluidics has led to significant improvements in many areas of in vitro diagnostics. This thesis proposes the use of microfluidic device technology in the field of anatomic pathology, leading to faster and more accurate tissue-based ...
EPFL2016A microfluidic device for longitudinal studies of C.elegans neurodegenerative disease models
Johan Auwerx, Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Laurent Mouchiroud, Govindan Narayanan Krishnamani
We describe a microfluidic device for automated culture and long-term high resolution imaging of Caenorhabditis elegans nematodes, which we specifically employed as model organisms for the analysis of neurodegenerative disease progression. In this platform ...
2015Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells
Philippe Renaud, Harald Van Lintel, Nina Buffi
We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentrat ...
Amer Inst Physics2014Programming and use of Parylene C fluorescence as a quantitative on-chip reference
Martinus Gijs, Diego Gabriel Dupouy, Ata Tuna Ciftlik, Joan Teixidor
A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...
Royal Soc Chemistry2014