Publication
Electron Capture Dissociation Product Ion Abundances at the X Amino Acid in RAAAA-X-AAAAK Peptides Correlate with Amino Acid Polarity and Radical Stability
Publications associées (79)
Improving binding affinity and stability of peptide ligands by substituting glycines with D-amino acids
Christian Heinis, Petr Leiman, David Gfeller, Shiyu Chen, Sergii Buth, Olivier Michielin
Improving the binding affinity and/or stability of peptide ligands often requires testing of large numbers of variants to identify beneficial mutations. Herein we propose a type of mutation that promises a high success rate. In a bicyclic peptide inhibitor ...
2013Novel Approaches in Tandem Mass Spectrometry for Peptide and Protein Structure Analysis
Results of the mechanistic studies of free-electron based fragmentation methods addressing peptide and protein structure analysis with mass spectrometry (MS), electron capture dissociation (ECD) and electron ionization dissociation (EID), are presented. Se ...
EPFL2012Multiple Isomers and Protonation Sites of the Phenylalanine/Serine Dimer
Our investigation of the phenylalanine/serine (Phe/Ser) protonated dimer suggests that the intermolecular interaction between the two amino acids is more complex than could have been anticipated from previous studies of similar systems. Isomer-specific inf ...
2012Peptide Structure and Radical Chemistry in the Gas Phase Revealed by Product Ion Abundance Analysis in Electron Capture and Transfer Dissociation Mass Spectrometry
Peptide and protein ion abundances in mass spectrometry (MS) and tandem mass spectrometry (MS/MS) may provide orthogonal, or complementary, information to their mass measurements. Collision induced dissociation (CID), the most commonly used MS/MS technique ...
EPFL2011Glycopeptide dendrimers: Tuning carbohydrate-lectin interactions with amino acids
Ronan Euzen, Jean-Louis Reymond
Glycodendrimers, such as glycoclusters and glycopolymers, are known to be very useful molecules to probe carbohydrate-lectin interactions. Herein, new second generation glycopeptide dendrimers (G2a-f) presenting a L-lysin-based (Lys) tetraantennary scaffol ...
Royal Society of Chemistry2011Visualization of proprotein convertase activity in living cells and tissues
The invention relates to a system for detecting the presence of a convertase cleaving activity, the system comprising a molecule comprising a proteolytic cleavage site with the amino acid sequence -R-X1-X2-R-, wherein R represents the amino acid arginine, ...
2011Radical Stability Directs Electron Capture and Transfer Dissociation of Beta-Amino Acids in Peptides
Yury Tsybin, Hisham Ben Hamidane, Aleksey Vorobyev
We report on the characteristics of the radical-ion-driven dissociation of a diverse array of beta-amino acids incorporated into alpha-peptides, as probed by tandem electron-capture and electron-transfer dissociation (ECD/ETD) mass spectrometry. The report ...
2010Conformation of Polyalanine and Polyglycine Dications in the Gas Phase: Insight from Ion Mobility Spectrometry and Replica-Exchange Molecular Dynamics
Yury Tsybin, Aleksey Vorobyev, Rodolphe Antoine
The conformation of model Arg(Ala)(4)X(Ala)(4)Lys+2H and Arg(Gly)(4)X(Gly)(4)Lys+2H peptides has been systematically investigated as a function of the central amino acid X through a combined experimental and theoretical approach. Mass spectrome ...
American Chemical Society2010Life-times of long-lived coherences under different motional regimes
Geoffrey Bodenhausen, Aurélien Bornet, Riddhiman Sarkar
The transverse relaxation rate R-2 of single quantum coherences, the relaxation rate R-LLC of long-lived coherences (LLC), and the ratio R-2/R-LLC have been studied by experiment, simulation and theory in the two-spin system formed by the Glycine aliphatic ...
2010Synthetic Studies on (-)-Lemonomycin: An Efficient Asymmetric Synthesis of Lemonomycinone Amide
Asym. synthesis of lemonomycinone amide was accomplished from readily accessible starting materials. Enantioselective alkylation of N-(diphenylmethylene)glycine tert-Bu ester by 5-tert-butyldimethylsilyloxy-2,4-dimethoxy-3-methylbenzyl bromide in the prese ...
2009