Publications associées à Nucleoprotein complexes of minute virus of mice have a distinct structure different from that of chromatin

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

Suliana Manley, Chen Zhang, Laurent Casini

DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial specie ...

Nature Portfolio2024

Molecular mechanism of plasmid elimination by the DdmDE defense system

Melanie Blokesch, Sandrine Stutzmann, David William Adams, Laurie Righi

Seventh pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryogenic electron microscopy to reveal the mechanistic basis for plasmid defense by DdmDE. A structure of the DdmD ...

2024

A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring

Sebastian Maerkl, Nicolas Rémi Adam, Jonathan Selz

PremiseA novel protocol for rapid plant DNA extraction using microneedles is proposed, which supports botanic surveys, taxonomy, and systematics. This protocol can be conducted in the field with limited laboratory skills and equipment. The protocol is vali ...

WILEY2023

C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System

Sebastian Maerkl, Laura Sophie Grasemann

Rolling circle amplification (RCA) is a widely used DNA amplification method that uses circular template DNA as input and produces multimeric, linear single- or double-stranded DNA. Circle-to-circle amplification (C2CA) has further expanded this method by ...

Washington2023

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Sandrine Gerber, Mounir Driss Mensi, Perrine Agnes Edith Robin, Alireza Kavand, Lucas Mayoraz

The COVID-19 pandemic has highlighted the necessity to develop fast, highly sensitive and selective virus detection methods. Surface-based DNA-biosensors are interesting candidates for this purpose. Functionalization of solid substrates with DNA must be pr ...

2023

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Martinus Gijs, Thomas Lehnert, Lin Sun

Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...

ROYAL SOC CHEMISTRY2022

Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools

Henning Paul-Julius Stahlberg, Dongchun Ni

Type I CRISPR-Cas systems typically rely on a two-step process to degrade DNA. First, an RNA-guided complex named Cascade identifies the complementary DNA target. The helicase-nuclease fusion enzyme Cas3 is then recruited in trans for processive DNA degrad ...

CELL PRESS2022

m6A methylation potentiates cytosolic dsDNA recognition in a sequence-specific manner

Andrea Ablasser, Muhammet Fatih Gülen

Nucleic acid sensing through pattern recognition receptors is critical for immune recognition of microbial infections. Microbial DNA is frequently methylated at the N-6 position of adenines (m6A), a modification that is rare in mammalian host DNA. We show ...

ROYAL SOC2021

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Nucleoprotein complexes of minute virus of mice have a distinct structure different from that of chromatin

Microsecond Time-Resolved Cryo-Electron Microscopy

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

Molecular mechanism of plasmid elimination by the DdmDE defense system

Regulation and impact of TERRA R-loops at human telomeres

A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring

C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools

m6A methylation potentiates cytosolic dsDNA recognition in a sequence-specific manner

Graph Chatbot

Chattez avec Graph Search

Microsecond Time-Resolved Cryo-Electron Microscopy

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

Regulation and impact of TERRA R-loops at human telomeres

C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System

A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Molecular mechanism of plasmid elimination by the DdmDE defense system

m6A methylation potentiates cytosolic dsDNA recognition in a sequence-specific manner