Monitoring bone fetal cells seeded in PLA scaffolds using bioluminescence imaging
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in vivo bioluminescence imaging (BLi) is an optical molecular imaging technique used to visualize molecular and cellular processes in health and diseases and to follow the fate of cells with high sensitivity using luciferase-based gene reporters. The high ...
Cellular heterogeneity is ubiquitous in any cell population. Understanding these variations is key to answering fundamental questions in immunology, developmental biology, cancer or stem cell biology. For instance, single-cell in vitro and in vivo assays h ...
Once a bone substitute, previously seeded with human bone progenitor cells, is implanted in a femoral condyle of a rat, it’s of importance to determine the intensity of a cell-triggered immune response of the host. Different techniques can be used, such as ...
By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...
The invention relates to a technique to detect small molecules using Bioluminescence imaging (BLI) to image and quantify non-invasively, in vitro and in vivo,intracellular metabolite fluxes and which can be applied to azido-modified compounds, such as azid ...
The present invention relates to a method for purging ex vivo isolated organs or isolated cells from latent human cytomegalovirus (HCMV). The invention further relates to a compound for use in a method for treating HCMV infection. ...
Transplantation of donor cornea is widely performed with a high success rate to treat patients with impaired corneal transparency. However the limited number of donors has led scientists to search for new technologies and treatments, including stem cell th ...
Regenerative medicine aims to replace or regenerate tissues or organs to re-establish their normal function. In 1975, Rheinwald and Green developed a technique to isolate and amplify epidermal stem cells. Their discovery led to the development of cultured ...
Stem cell therapies hold tremendous potential for tissue and organ regeneration. Yet, there remains significant need for better ex vivo culture and manipulation methods. On the one hand, many tissue-specific stem cells cannot be propagated without causing ...
Bone fracture healing problems, because of the loss of tissue regeneration, can affect many old or multi-pathologic patients around the world. Among the therapeutic strategies, bone substitutes, such as poly-(L-lactitve acid) with 5% wt. % ß-tricalcium pho ...