Êtes-vous un étudiant de l'EPFL à la recherche d'un projet de semestre?
Travaillez avec nous sur des projets en science des données et en visualisation, et déployez votre projet sous forme d'application sur Graph Search.
Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted electrostatic self-assembly. Hereafter, they were grafted by exposure to a solution of 5D10 monoclonal antibodies (mAb) and fibronectin (FN), with the first being used for immunospecific cell capture and the latter being used for cell adhesion and growth. A solution of target MCF-7 cells mixed with Jurkat cells was brought inside the microchannel, leading to specific MCF-7 cell capture; the latter were then cultured and evidenced by cell immuno-luminescence.
Philippe Renaud, Arnaud Bertsch, Clémentine Sophie Sarah Lipp
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz