Fluorescence correlation spectroscopy with sub-diffraction-limited resolution using near-field optical probes
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The aim of the present PhD thesis is the elaboration of new, home-made Scanning Near-field Optical Microscope (SNOM or NSOM), and the demonstration of its great potential for high-resolution topography and fluorescence investigations of soft and solid samp ...
We report the growth of ultrasharp carbon whiskers onto apertured near-field optical glass fiber probes. The ultrasharp carbon whiskers are produced by the electron-assisted dissociation of residual oil vapors present in the vacuum chamber during the elect ...
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Life sciences have a constantly growing need for novel methodological approaches suitable to investigate the intracellular environment with increased temporal and spatial resolutions. Recently, optical near-field probes, such as laser-irradiated pointed me ...
The need to image objects on increasingly finer scales and spatially localized specific molecules can be met by the combination of infrared, visible and Raman spectroscopy with scanning near-field microscopy, giving rise to a powerful nanospectroscopic too ...
A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor?ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. T ...
The combination of the topographic resolution of Scanning Probe Microscopy with the subwavelength information of light/matter interaction leads to a new instrument called Scanning Near-field Optical Microscope (SNOM). This new instrument is an ideal tool t ...
Reflection digital holographic microscopy (DHM) is a very powerful technique allowing measuring topography with a sub-nanometer axial resolution from a single hologram acquisition. But as most of interferometer methods, the vertical range is limited to hal ...
Near-field optical probes have been used to produce a subdiffraction-limited ob- servation area for fluorescence correlation spectroscopy (FCS) experiments on supported membranes. The design of a bent, etched fiber probe that is compatible with biological ...
Stimulated emission depletion (STED) resolves fluorescent features that are closer than the far-field optical diffraction limit by applying a spatially modulated light field keeping all but one of these features dark consecutively. For estimating the effic ...
We report on the fabrication and characterization of nanoscale solid immersion lenses (nano-SILs) with sizes down to a subwavelength range. Submicrometer-scale cylinders fabricated by electron-beam lithography are thermally reflowed to form a spherical sha ...
