Publication
An immune-responsive Serpin regulates the melanization cascade in Drosophila
Publications associées (34)
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We developed a rational approach to design peptide-based covalent inhibitors and coupled the inhibitors with antibodies for cell-specific delivery. We used this platform to generate antibody-peptide inhibitor conjugates (APICs) that target a family of prot ...
Nature Portfolio2024Development of cyclic peptide inhibitors of coagulation factor XIa for safer anticoagulation
Hemostasis is a complex physiological process responsible for the prevention of blood loss caused by vascular injury. The dysregulation of this delicate mechanism can lead to thromboembolic diseases, the leading cause of death worldwide. Anticoagulants are ...
EPFL2019More Than Black or White: Melanization and Toll Share Regulatory Serine Proteases in Drosophila
Bruno Lemaitre, Jan Paul Dudzic, Mark Austin Hanson, Igor Iatsenko
The melanization response is an important defense mechanism in arthropods. This reaction is mediated by phenoloxidases (POs), which are activated by complex extracellular serine protease (SP) cascades. Here, we investigate the role of SPs in the melanizati ...
CELL PRESS2019The melanization reaction in Drosophila: More than black or white
The melanization reaction is a rapid and important immune mechanism in arthropods. It results in the production of melanin at the site of injury and around invading microbes. The enzymes responsible for melanogenesis are phenoloxidases (PO), which catalyze ...
EPFL2018Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps
Bruno Lemaitre, Samuel Rommelaere, Anna Dostalova
Background: Members of the thioester-containing protein (TEP) family contribute to host defence in both insects and mammals. However, their role in the immune response of Drosophila is elusive. In this study, we address the role of TEPs in Drosophila immun ...
2017Middle-down approaches for mass spectrometry-based protein identification and characterization
Mass spectrometry (MS) has emerged over the last two decades as the analytical technique of choice in systems-level protein studies, known as proteomics. Two are the MS-based approaches generally applied to proteomics: bottom-up (BU), which relies on the p ...
EPFL2016The Black cells phenotype is caused by a point mutation in the Drosophila pro-phenoloxidase 1 gene that triggers melanization and hematopoietic defects
Bruno Lemaitre, Maroun Bou Sleiman, Olivier Binggeli, Lise Bertin
Melanization contributes to arthropod-specific innate immunity through deposition of melanin at wound sites or around parasites, with concomitant release of microbicidal reactive oxygen species. Melanization requires sequential activation of proteolytic en ...
Elsevier2015Prophenoloxidase Activation Is Required for Survival to Microbial Infections in Drosophila
Bruno Lemaitre, Olivier Binggeli
The melanization reaction is a major immune response in Arthropods and involves the rapid synthesis of melanin at the site of infection and injury. A key enzyme in the melanization process is phenoloxidase (PO), which catalyzes the oxidation of phenols to ...
Public Library of Science2014Methods to study Drosophila immunity
Bruno Lemaitre, Olivier Binggeli, Andrew Jonathan Bretscher
Innate immune mechanisms are well conserved throughout evolution, and many theoretical concepts, molecular pathways and gene networks are applicable to invertebrate model organisms as much as vertebrate ones. Drosophila immunity research benefits from an e ...
Elsevier2014Role and regulation of the melanization reaction in Drosophila immune response
In arthropods, the melanization reaction consists in the rapid synthesis of melanin at the site of infection and injury. A key enzyme in the melanization process is phenoloxidase (PO), which catalyzes the oxidation of phenols to quinones that subsequently ...
EPFL2013