Turn-Off-and-On: Chemosensing Ensembles for Sensing Chloride in Water by Fluorescence Spectroscopy
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A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. it relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct ...
To study the physicochemical properties of the DTTA chelating moiety (H4DTTA = diethylenetriaminetetraacetic acid = N,N′-[iminobis(ethane-2,1-diyl)]bis[N-(carboxymethyl)glycine]), used in several compounds proposed as magnetic resonance imaging (MRI) contr ...
This Ph.D. work deals with the development of lanthanide-based bioprobes for biomedical analyses and imaging. For this purpose, two different approaches have been followed, one focusing on visible-emitting complexes, while the second addresses the sensitiz ...
Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...
12-Metallacrown-3 complexes were formed by self-assembly of organometallic half-sandwich complexes [(π-ligand)MCl2]2 (M = Ru, Rh, Ir) and 3-hydroxy-2-pyridone ligands in the presence of Cs2CO3 as base. Upon mixing of different macrocycles, reversible metal ...
New polydentate ligands (e.g., Tsox and TsoxMe) have been synthesized to take advantage of the chelating effect of bidentate 8-hydroxyquinolinate subunits connected to a N,N,N¢,N¢-tetraaminopropyl-1,2-ethylenediamine framework and with the aim of sensitizi ...
The aim of this thesis is to optimise and gain fundamental information on two applications of photomedicine using fluorescence imaging and spectrofluorometry: (1) the detection of early bronchial cancer by autofluorescence imaging and (2) the endometrial a ...
The first observation of electrochemiluminescence (ecl) based on the redox-cycling of a ruthenium chelate covalently linked to a protein and placed in aqueous medium containing neither solvent nor reducing compound is reported here. Ecl experiments based o ...
Autofluorescence is emerging as a useful tool for the detection of early cancers in the bronchi. It has already produced interesting results, which have been implemented in commercial imaging devices. Their design relies on the spectroscopy of the tissues ...
Novel fluorescence approaches to investigate ligand recognition and structure of G protein-coupled receptors in native membranes have been developed. These methods combine the biosynthetic incorporation of unnatural fluorescent amino acids at known sites i ...