Neurite guidance through 3D hydrogel layers in a microfluidic environment
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versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to gener ...
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With a growing number of cell-based biotechnological applications, there is a need for particle separation systems capable of multiparameter separations at high purity and throughput, beyond what is presently offered by traditional methods including fluore ...
We present a simple and easy to handle PDMS microfluidic device for neuronal cell culture studies in three-dimensional hydrogel scaffolds. The hydrogel is structured in parallel layers to reconstruct cell layers close to the natural environment. Dissociate ...
Specific-purpose microfluidic devices have had considerable impact on the biological and chemical sciences, yet their use has largely remained limited to specialized laboratories. Here we present a general-purpose software-programmable microfluidic device ...
By enhancing the environment of micro electrode arrays with microfluidic channels we cultured primary neuronal cells in patterned two- and three dimensional structures. Alternatively, the same microfluidic channels can be used to generate a local chemical ...
[...] Studying axon guidance by diffusible or substrate bound gradients is challenging with current techniques. In this study, we present two microfluidic devices to study axon guidance. [... click on "Download fulltext" for full abstract] ...
This thesis reports on the integration of continuous-flow cell separation method for lab-on-a-chip applications. Cell separation methods are widely used in biology to prepare samples prior to analysis. There is a need for a highly sensitive separation meth ...
Since the introduction of the of Micro Total Analysis Systems concept in 1990, important progress in microfluidic analytical systems has been realized. In many cases, microfluidic systems have shown improved analytical performances over classical methods, ...
The physiological properties of cells are typically investigated in ensembles yielding averaged data that mask heterogeneities present in any cell population. Single-cell analysis techniques based on microfluidic "lab on a chip" (LOC) devices have recently ...
