Kinetics of antimicrobial peptide activity measured on individual bacterial cells using high-speed atomic force microscopy
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Reducing the emergence and spread of antibiotic-resistant bacteria is one of the major healthcare issues of our century. In addition to the increased mortality, infections caused by multi-resistant bacteria drastically enhance the healthcare costs, mainly ...
ATP is a key molecule of cell physiology, but despite its importance, there are currently no methods for monitoring single-cell ATP fluctuations in live bacteria. This is a major obstacle in studies of bacterial energy metabolism, because there is a growin ...
My PhD thesis aims to obtain the structure of several bacteriophages, including so-called “jumbo” phages, using the electron cryo-microscopy (cryo-EM) and to study the structural transformation of these viruses as they attach and infect the host cell. We a ...
In the latest years the importance of high resolution analysis of the microbial cell surface has been increasingly recognized. Indeed, in order to better understand bacterial physiology and achieve rapid diagnostic and treatment techniques, a thorough inve ...
By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...
In this article, we introduce the real-time cellular force microscope (RT-CFM), a high-throughput microrobotic platform for mechanical stimulation and characterization of single cells. We developed computer vision algorithms that fully automate the positio ...
The widespread misuse of drugs has increased the number of multiresistant bacteria1, and this means that tools that can rapidly detect and characterize bacterial response to antibiotics are much needed in the management of infections. Various techniques, s ...
Persistent cells, representing a small fraction of a bacterial population, are refractory to antibiotic therapy. It is a phenotypic transient state. The underlying mechanisms of persistence remain largely unknown, but even divergent hypotheses agree on a f ...
The increasing threat of multidrug-resistant bacterial strains against conventional antibiotic therapies represents a significant worldwide health risk and intensifies the need for novel antibacterial treatments. In this work, an effective strategy to targ ...
We present here a technological platform for engineering Au nanotopographies by templated electrodeposition for antibacterial surfaces. Three different types of nanostructures were fabricated: nanopillars, nanorings and nanonuggets. The nanopillars are the ...