Publication
Lineage- and stage-restricted lentiviral vectors for the gene therapy of chronic granulomatous disease
Publications associées (78)
Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics
Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...
EPFL2024Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures
Kai Johnsson, Yann Barrandon, Johannes Alexander Mosig, Thomas Michael Braschler, Ariane Rochat, Jean-Baptiste Bureau, Fahd Azzabi Zouraq, Mako Kamiya
Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate ...
WILEY2023HTATIP2 regulates arteriogenic activity in monocytes from patients with limb ischemia
Michele De Palma, Mario Leonardo Squadrito
Use of autologous cells isolated from elderly patients with multiple comorbidities may account for the modest efficacy of cell therapy in patients with chronic limb threatening ischemia (CLTI). We aimed to determine whether proarteriogenic monocyte/macroph ...
Ann Arbor2023PD-1-cis IL-2R agonism yields better effectors from stem-like CD8(+) T cells
Douglas Hanahan, Mélanie Louise Tichet, Petra Catalina Schwalie, Stephan Wullschleger, Maria Karagianni
Expansion and differentiation of antigen-experienced PD-1(+)TCF-1(+) stem-like CD8(+) T cells into effector cells is critical for the success of immunotherapies based on PD-1 blockade(1-4). Hashimoto et al. have shown that, in chronic infections, administr ...
NATURE PORTFOLIO2022Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions
Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...
EPFL2022Efficient viral transduction in mouse inner ear hair cells with utricle injection and AAV9-PHP.B
Bernard Schneider, Paola Andrea Solanes Vega
Viral delivery of exogenous coding sequences into the inner ear has the potential for therapeutic benefit for patients suffering genetic or acquired hearing loss. To devise improved strategies for viral delivery, we investigated two injection techniques, r ...
2020The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells
Precise spatiotemporal regulation of gene expression is essential for development and homeostasis of complex organisms. This is achieved in large part by sequence-specific transcription factors (TF) that bind to genomic regulatory elements to activate or r ...
EPFL2019Bioengineering the Human Muscle Stem-Cell Niche for Therapeutic Applications
In spite of decades of research, no feasible method for obtaining sufficient numbers of uncommitted muscle stem cells (MuSCs) for therapy of degenerative muscle diseases exists. One of the most fundamental problems associated with stem cell therapy of musc ...
EPFL2019Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells
Matthias Lütolf, Felix Naef, Yoji Tabata, Eric Paquet, François Réal Rivest, Ambroise Roger Vuaridel, Andrea Manfrin
Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...
NATURE PUBLISHING GROUP2019Memory and relatedness of transcriptional activity in mammalian cell lineages
Felix Naef, David Michael Suter, Nicholas Edward Phillips, Aleksandra Mandic, Saeed Omidi Klishami
Phenotypically identical mammalian cells often display considerable variability in transcript levels of individual genes. How transcriptional activity propagates in cell lineages, and how this varies across genes is poorly understood. Here we combine live- ...
2019