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Automated Detection and Segmentation of Synaptic Contacts in Nearly Isotropic Serial Electron Microscopy Images

Publications associées (37)

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Efficient scanning for em based target localization

Pascal Fua, Graham Knott, Raphael Sznitman, Aurélien Lucchi

We presented an approach for speeding-up image acquisition when tasked with localizing specific structures in FIB-SEM imagery. It exploits the fact that low-quality images can be acquired faster than higher-quality ones and yet be sufficient for inference ...

2014

Development of sol-gel-derived multi-wall carbon nanotube/hydroxyapatite nanocomposite powders for bone substitution

Hossein Najafi

Carbon nanotubes with unique physical and mechanical properties have shown great potential for biological applications, including tissue engineering and mimicking the structure and properties of human bones. In the present work, sol-gel synthesized nanocom ...

Sage Publications Ltd2014

SHPM imaging of LiHoF4 at ultra low temperatures

In the first part of this thesis we describe the functioning principle of the scanning hall probe microscope (SHPM), and we present a summary of the theoretical and experimental knowledge on the dipolar-coupled Ising ferromagnet LiHoF4. In the second chapt ...

2014

SHPM imaging of LiHoF4 at ultra low temperatures

2014

Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons

Marco Cantoni, Graham Knott, Bohumil Maco

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, pre ...

Public Library of Science2013

Universal sample holder

Graham Knott

This device is for holding samples during their preparation prior to imaging in the electron microscope. The design means it can be transferred between the light and electron microscopes as well as trimming devices used to prepare the final sample. It can ...

2013

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Aleksandra Radenovic, Lorenz Jan Steinbock

The ability to reshape nanopores and observe their shrinkage under an electron microscope is a powerful and novel technique. It increases the sensitivity of the resistive pulse sensing and enables to detect very short and small molecules. However, this has ...

American Chemical Society2013

Three Dimensional Microanalysis by Energy Dispersive Spectrometry

Pierre Burdet

This thesis is focused on a combined microscopy technique: energy dispersive spectrometry (EDS) is extended to a three dimensional (3D) microanalysis using a so-called "dual-beam microscope": a scanning electron microscope (SEM) equipped with a focused ion ...

EPFL2012

Structured Image Segmentation using Kernelized Features

Pascal Fua, Kevin Smith, Aurélien Lucchi, Yunpeng Li

Most state-of-the-art approaches to image segmentation formulate the problem using Conditional Random Fields. These models typically include a unary term and a pairwise term, whose parameters must be carefully chosen for optimal performance. Recently, stru ...

2012

Nonlinear phase contrast enhancement

Demetri Psaltis, Alexandre Goy

We demonstrate a simple method to detect small phase steps using propagation in Kerr media. In experiments, nonlinear diffraction in acetone produced high contrast signals for a pi/80 phase mask. (C) 2010 Optical Society of America ...

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