Parallel AFM imaging and force spectroscopy using two-dimensional probe arrays for applications in cell biology
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The goals of tissue engineering include recapitulating specific tissue functions for regenerative medicine and developing in vitro models of human tissues to study human physiology and pathophysiology and for testing and screening drugs before expensive cl ...
Atomic force microscopy has become potent tool for investigating interand intramolecular interactions. Single molecule force spectroscopy on supramolecular guest-host-complexes reveal information about the depth of the binding pocket and thermal off-rates. ...
Tendons are composed of collagen and other molecules in a highly organized hierarchical assembly, leading to extraordinary mechanical properties. To probe the cross-links on the lower level of organization, we used a cantilever to pull substructures out of ...
‘Real-time’ nanoscale imaging and single molecule force spectroscopy of bio-chemical specimen based on atomic force microscopy (AFM) requires cantilevers with both low force constant and high-resonant frequency. The required spring constant should be betwe ...
The thesis presents the development of a custom made atomic force microscope (AFM) conceived to image biological molecules at low temperatures in ultrahigh vacuum conditions, and its application to DNA single molecule studies. Starting from a first prototy ...
The fracture resistance of biomineralized tissues such as bone, dentin, and abalone is greatly enhanced through the nanoscale interactions of stiff inorganic mineral components with soft organic adhesive components. A proper understanding of the interactio ...
We present the fabrication and characterization of hybrid SPM probes with full platinum (Pt) cantilever and tip for biological SPM experiments. In recent years, SPM has shown its value in imaging and characterizing biological samples. Classic SPM sensors f ...
Sacrificial bonds and hidden length in structural molecules and composites have been found to greatly increase the fracture toughness of biomaterials by providing a reversible, molecular-scale energy-dissipation mechanism. This mechanism relies on the ener ...
Experimental and numerical investigations were carried out on adhesively bonded full-scale double lap joints composed of pultruded GFRP profiles with relatively thick adhesive layers. Thick adhesive layers are often used in infrastructure applications in o ...
The use of force spectroscopy to study the adhesion of living fibroblasts to their culture substrate was investigated. Both primary fibroblasts (PEMF) and a continuous cell line (3T3) were studied on quartz surfaces. Using a fibronectin- coated AFM cantile ...