2D Stem Cell Culture on Microfluidic Generated Hydrogel Beads
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According to the last WHO numbers, cardiac diseases remain the first cause of death in the world. Cell therapy represents a promising approach to restore heart function. Cardiac derived adherent proliferation (CAP) cells were shown to support the heart reg ...
An estimated 400 million people worldwide suffer from bladder diseases. However, little is known at the cellular level as to what causes or initiates many of these diseases. Therefore, this thesis has focused on understanding one cell type within the bladd ...
Microwell arrays are cell culture and imaging platforms to assess cells at a single cell level and in high-throughput. They allow the spatial confinement of single cells in microfabricated cavities on a substrate and thus the continuous long-term observati ...
Mesenchymal Stem Cells (MSC) are multipotent stem cells that are predominantly obtained from the bone marrow. These cells have a high ability to differentiate into a multitude of cell types, which are very attractive for tissue engineering and cell therapy ...
Mechanical stimuli have fundamental repercussions on a cell’s phenotype and response in vivo. Studying these effects in a controlled in vitro bioreactor system allows bioengineers to hone in on the regenerative potential of therapeutic cells to better desi ...
Biosensors have been applied to disparate fields, especially for endogenous compounds such as glucose and lactate. The main areas of application are certainly related to medical and diagnostic purposes. However, metabolic monitoring can be also of interest ...
Articular cartilage regeneration poses particularly tough challenges for implementing cell-‐based therapies. Many cell types have been investigated looking for a balanced combination of responsiveness and stability, yet techniques are still far from defin ...
Of the many external factors that affect cell behavior, mechanical cues are fundamental in modulating a cell’s phenotype. Biomimetic mechanosensing requires the three-dimensional encapsulation of cells within a controlled microenvironment. As musculoskelet ...
We present the microrheological study of the two close human epithelial cell lines: non-cancerous HCV29 and cancerous T24. The optical tweezers tracking was applied to extract the several seconds long trajectories of endogenous lipid granules at time step ...
Hematopoietic stem cells (HSCs) have a unique ability to self-renew and produce differentiated progeny throughout the entire life of the human body. However, present clinical use of HSCs in regenerative medicine is largely hindered by our limited understan ...