Publication

Anthracene-Tethered Ruthenium(II) Arene Complexes as Tools To Visualize the Cellular Localization of Putative Organometallic Anticancer Compounds

Publications associées (33)

Surface passivation and functionalisation for mass photometry

Suliana Manley, Jenny Sülzle, Laila Abdelaziz Abdelmoniem Elfeky

Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...

Wiley2024

Fluorescence microscopy: A statistics-optics perspective

Aleksandra Radenovic

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scal ...

Amer Physical Soc2024

Event-driven acquisition for content-enriched microscopy

Suliana Manley, Martin Weigert, Chen Zhang, Willi Leopold Stepp, Dora Mahecic, Juliette Griffie

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

NATURE PORTFOLIO2022

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Michael Stefan Daniel Liebling, Christian Jaques

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Michelle Susanne Frei

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

EPFL2020

Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy

Christian Jaques

Light microscopy is a tool of paramount importance for biologists and has been constantly improved for the past four centuries. Despite many recent developments, microscopy techniques still require improvement, especially to reach better temporal and spect ...

EPFL2020

Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

Adrien Charles-François Raymond Descloux

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy

Esther Amstad, Stefan Matile, Maria Tsemperouli

Horizontal black lipid membranes (BLMs) enable optical microscopy to be combined with the electrophysiological measurements for studying ion channels, peptide pores, and ionophores. However, a careful literature review reveals that simultaneous fluorescenc ...

AMER CHEMICAL SOC2019

Photons in - numbers out: perspectives in quantitative fluorescence microscopy for in situ protein counting

Kristin Stefanie Grussmayer

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein assemblies and cellu ...

2019

Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)

Ardemis Anoush Boghossian, Benjamin Paul Johanès Gabriel Lambert, Alessandra Antonucci, Vitalijs Zubkovs

Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000–1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as negligible tissue autofluorescence w ...

2018

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