Visualization of proteins in intact cells with a clonable tag for electron microscopy
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Cryo-electron microscopy is a form of transmission electron microscopy that has been used to determine the 3D structure of biological specimens in the hydrated state and with high resolution. We report the development of 4D cryo-electron microscopy by inte ...
Electron crystallography is used to study membrane proteins in the form of planar, two-dimensional (2D) crystals, or other crystalline arrays such as tubular crystals. This method has been used to determine the atomic resolution structures of bacteriorhodo ...
Since electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed by ...
New evidence is reported for TiON sputtered polyester surfaces activated by sunlight irradiation leading to the accelerated bacterial inactivation in the minute range. The absorption in Kubelka-Munk units of the TiON film was observed to be directly propor ...
A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic wr ...
The ability to observe axons and dendrites with transmission electron microscopy (EM) after they have been previously imaged live with laser-scanning microscopy is a useful technique to study their synaptic connectivity. This protocol provides a detailed m ...
LiFePO4 and FePO4 phase distributions of entire cross-sectioned electrodes with various Li content are investigated from nanoscale to mesoscale, by transmission electron microscopy and by the new electron forward scattering diffraction technique. The distr ...
The great achievement of nanotechnology is the controlled synthesis of a large variety of nanometer-size materials like needle-formed nanotubes, nanowires and two-dimensional graphene flakes. Due to their unique physico-chemical properties, these nanostruc ...
The transition between different states in manganites can be driven by various external stimuli. Controlling these transitions with light opens the possibility to investigate the microscopic path through which they evolve. We performed femtosecond (fs) tra ...
Aim: Ultrasmall superparamagnetic iron oxide nanoparticles (USPIO-NPs) are under development for imaging and drug delivery; however, their interaction with human blood brain barrier models is not known. Materials & Methods: The uptake, reactive oxygen spec ...