The molecular mechanisms of antimetastatic ruthenium compounds explored through DIGE proteomics
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The different steps associated with the curing of a PVAc/polyester blend are identified and correlated to the mechanism of shrinkage control in the presence of a low-profile additive (LPA). Poly(vinyl acetate) (PVAc) is used as a LPA and is shown to induce ...
The protein binding of anticancer metallodrugs is regarded as an important part in their mode of action both for delivering the active moiety into the tumor but also being responsible for deactivation and/or unwanted side effects. Characterization of prote ...
Membrane-associated proteins of Mycobacterium tuberculosis offer a challenge, as well as an opportunity, in the quest for better therapeutic and prophylactic interventions against tuberculosis. The authors have previously reported that extraction with the ...
Isoelectric focusing in a polyacrylamide pH gradient gel is used to analyze the size distribution of gold nanoparticles synthesized by a chemical route with mercaptosuccinic acid as a ligand. The isoelectric point of the nanoparticles is shown to be size d ...
The Off-Gel™ technology is an isoelectric focusing (IEF) fractionation method which enables the purification of one or several proteins in a solution placed on an immobilised pH gradient (IPG) gel. The present thesis is devoted to its characterisation and ...
Bacteriophage T4 makes a large number of prereplicative proteins, which are involved in directing the transition from host to phage functions, in producing the new T4 DNA, and in regulating transcriptional shifts. We have used two-dimensional gel electroph ...
The aim of this study was to characterize mammalian glycosyl phosphatidylinositol (GPI)-anchored proteins y two-dimensional gel electrophoresis using immobilized pH gradients. Analysis was performed on detergent-resistant membrane fractions of baby hamster ...
Export of mRNA from the nucleus to the cytoplasm was studied in mature Xenopus laevis oocytes. In vitro transcribed, capped 32P-labeled mRNA was microinjected into nuclei, and its appearance in the cytoplasm measured by counting radioactivity or by RNA ext ...
