Fingerprinting the Tertiary Structure of Electroadsorbed Lysozyme at Soft Interfaces by Electrostatic Spray Ionization Mass Spectrometry

Lysozyme can be electrochemically detected after adsorption at an electrified gel-water interface. Ex-situ characterization by electrostatic spray ionization mass spectrometry provides insights into the interfacial detection mechanism by allowing changes to the tertiary structure of electroadsorbed lysozyme to be fingerprinted for the first time.