Publication
New analytical tools combining gel electrophoresis and mass spectrometry
Publications associées (94)
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Glycans play a pivotal role in both physiological processes; however, the field of glycobiology remains relatively understudied within biochemistry. The intricate complexity of glycans, coupled with their abundance of isomeric forms, presents formidable ch ...
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ELSEVIER SCI LTD2022The role of cavitation in drying cementitious materials
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This study examines the role of cavitation in drying cementitious materials. Classical nucleation theory is used to understand and study the parameters that influence cavitation pressures. The water desorption isotherms indicate that both heterogeneous and ...
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EPFL2021A simple micropreparative gel electrophoresis technique for purification of proteins, nucleic acids, and bioconjugates
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The biochemical and biomedical fields hinge on the ability to effectively separate and purify biological macromolecules. Though this need is largely addressed with a variety of chromatographic and electrophoretic purification techniques, such techniques ar ...
2021Proteomics of Human Milk: Definition of a Discovery Workflow for Clinical Research Studies
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2021Online Aerosol Chemical Characterization by Extractive Electrospray Ionization-Ultrahigh-Resolution Mass Spectrometry (EESI-Orbitrap)
Current mass spectrometry techniques for the online measurement of organic aerosol (OA) composition are subjected to either thermal/ionization-induced artifacts or limited mass resolving power, hindering accurate molecular characterization. Here, we combin ...
AMER CHEMICAL SOC2020Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes
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Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...
2020Novel Analytical Methods for Allergy-Related Studies
Managing and treating diseases require a deep understanding of the pathologies coupled with accurate diagnostic procedures. The constant development and improvement of bioanalytical methods reflect the need of diagnosis ameliorations with respect to accura ...
EPFL2020Spectroscopic Evidence for Peptide-Bond-Selective Ultraviolet Photodissociation
Vladimir Kopysov, Aleksandr Pereverzev
We study the photodissociation induced by ultraviolet excitation of amide bonds in gas-phase protonated peptides. Jointly, mass spectrometry and cold ion spectroscopy provide evidence for a selective non-statistical dissociation of specific peptide bonds i ...
2020