Towards a kinetic model of the phospholipids biosynthesis of the yeast Saccharomyces cerevisiae
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Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...
Fundamental aspects of chemostat cultures are reviewed. Using yeast cultures as examples, it is shown that steady states in chemostats may be predicted quant. by combining the correct no. of unstructured kinetic models with expressions for existing stoichi ...
Yeast strains were examd. for their ability to produce glycerol, and Kluyveromyces marxianus NRRLY-665 was chosen for further optimization due to its comparatively high growth rate (0.15/h) and glycerol yield (9.5% by wt. on lactose) at 37 Deg and pH 7. Op ...
Yeasts degrade glucose through different metabolic pathways, where the choice of the pathway is dependent on the nature of the limitation in the various substrates. When oxygen is limiting in addn. to glucose, yeasts often grow according to a mixt. of oxid ...
The whi2 mutation in S. cerevisiae allows mutant cells to grow with a higher specific rate and at smaller cell sizes at the onset of EtOH metab. in diauxic growth. S. cerevisiae Whi2+ and whi2 were cultivated in aerobic and anaerobic batch cultures in the ...
A simple definition of the energetic growth efficiency both in terms of enthalpy and free energy is given and discussed. In cultures growing by respiratory catabolism, these efficiencies may conveniently be obsd. online by isothermal reaction calorimetry. ...
Chemostat cultures of an aerobic fermenting yeast (Saccharomyces cerevisiae) and an aerobic respiring yeast (Kluyveromyces fragilis) were grown under dual C + N limitation in an isothermal reaction calorimeter. The dual limitations resulted in uncoupled ox ...
To study the potential use of supercrit. CO2 extn. for the recovery of products from yeast cell cultures, the effects of CO2 on different yeast strains over a range of pressures and temps. have been examd. Viability was shown to be dependent on temp. and d ...
We have used monospecific antibodies against three ribonuclease H enzymes of Saccharomyces cerevisiae to investigate their intracellular localization. Fractionation experiments, as well as immunocytochemical staining, revealed a predominantly cytoplasmic l ...
In order to study the individual steps involved in the import of phosphatidylcholine (PC) into rat liver mitochondria, a number of PC analogues were introduced into the outer membrane of isolated mitochondria. Two fluorescent PC species, i.e. 1-palmitoyl-2 ...