Publication

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Publications associées (116)

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

EPFL2023

Microfluidic devices

Matthias Lütolf, Jisoo Park

The present disclosure relates to a microfluidic device comprising: a substrate; a culture chamber (130); a loading channel (170) in fluid communication with the culture chamber; at least one auxiliar channel (170-1, 5 170-2) extending from and in fluid co ...

2023

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

Martinus Gijs, Farzad Rezaeianaran

We study the presence within the worm Caenorhabditis elegans (C. elegans) of a fluorescent strain of the worm's bacterial food (Escherichia coli (E. coli) OP50) during early adulthood. Use of a microfluidic chip based on a thin glass coverslip substrate al ...

ROYAL SOC CHEMISTRY2023

Automated droplet manipulation in microfluidic systems

Christoph Merten, Jatin Panwar

Disclosed herein is a method of operating a microfluidic system, a microfluidic system for manipulating sample objects, and a control system for operating a microfluidic system. The method according to the invention is for operating a microfluidic system t ...

2023

In-flow electrochemical detection of chemicals in droplets with pyrolysed photoresist electrodes: application as a module for quantification of microsampled dopamine

Philippe Renaud, Arnaud Bertsch, Joan Teixidor, Albert René Yvon Leroy

The electrochemical quantification of analytes in droplets of PBS separated by a fluorinated phase was investigated. PDMS-fused silica chips with pyrolysed photoresist electrodes were prepared using a simple fabrication technique and used to analyze drople ...

ROYAL SOC CHEMISTRY2021

Microfluidic-Based Study on the Activation and Evolution of Calcite Bio-Mineralization for Geotechnical Applications

Lyesse Laloui, Dimitrios Terzis, Filippo Miele, Ariadni Elmaloglou

The field of geotechnics is moving fast towards benefiting from new methods and tools to improve the understanding of couplings among complex transport, chemical and biological phenomena. Calcite bio-mineralization, result of bio-grouting, stands out as su ...

2020

Facile Fabrication of Microfluidic Chips for 3D Hydrodynamic Focusing and Wet Spinning of Polymeric Fibers

Fabien Sorin, René M. Rossi, Efe Armagan, Claudio Toncelli, Akin Gürsoy

Microfluidic wet spinning has gained increasing interest in recent years as an alternative to conventional wet spinning by offering higher control in fiber morphology and a gateway for the development of multi-material fibers. Conventionally, microfluidic ...

2020

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

Carlotta Guiducci, Pierre-Emmanuel Marie Thiriet, Gloria Porro, Danashi Imani Medagoda

The simplicity of homogeneous immunoassays makes them suitable for diagnostics of acute conditions. Indeed, the absence of washing steps reduces the binding reaction duration and favors a rapid and compact device, a critical asset for patients experiencing ...

2020

A 3D printed wearable device for sweat analysis

Sandro Carrara, Mahshid Alsadat Padash

This work presents a wearable microfluidic device for sweat analysis. The purpose of this device is to collect sweat from the skin and convey it to the sensor. The device is printed by a 3D printer with Multi-Jet Modelling (MJM) technology using flexible m ...

IEEE2020

Studying the roundworm Caenorhabditis elegans using microfluidic chips

Martinus Gijs

C. elegans is an attractive model organism in biology, as it shows genetic similarity with humans, facilitates microscopic observation due to its transparency, and has a short life cycle. Moreover, many mutants expressing fluorescent proteins in particular ...

SPIE-INT SOC OPTICAL ENGINEERING2019

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