Publication
Harmonic Nanoparticles for Regenerative Research
Publications associées (34)
ISSCR standards for the use of human stem cells in basic research
The laboratory culture of human stem cells seeks to capture a cellular state as an in vitro surrogate of a biological system. For the results and outputs from this research to be accurate, meaning-ful, and durable, standards that ensure reproducibility and ...
Cambridge2023Programming of Multicellular Patterning with Mechano-Chemically Microstructured Cell Niches
Multicellular patterning of stem-cell-derived tissue models is commonly achieved via self-organizing activities triggered by exogenous morphogenetic stimuli. However, such tissue models are prone to stochastic behavior, limiting the reproducibility of cell ...
WILEY2023Generation of Human iPSC-Derived Neurons on Nanowire Arrays Featuring Varying Lengths, Pitches, and Diameters
Anna Fontcuberta i Morral, Wonjong Kim
Nanowire (NW) arrays interfaced with biological cells have been demonstrated to be potent tools for advanced applications such as sensing, stimulation, or drug delivery. Many implementations, however, have so far only been studied with rather robust basic ...
WILEY2022Primate-restricted KRAB zinc finger proteins and target retrotransposons control gene expression in human neurons
Didier Trono, Bart Deplancke, Priscilla Turelli, Christian Axel Wandall Thorball, Julien Léonard Duc, Charlène Mireille Raymonde Raclot, Alexandre Coudray, Julien Paul André Pontis, Delphine Yvette L Grun, Christopher James Playfoot, Evgeniia Pankevich
In the first days of embryogenesis, transposable element-embedded regulatory sequences (TEeRS) are silenced by Kruppel-associated box (KRAB) zinc finger proteins (KZFPs). Many TEeRS are subsequently co-opted in transcription networks, but how KZFPs influen ...
2020Marker-Free Automatic Quantification of Drug-Treated Cardiomyocytes with Digital Holographic Imaging
Gerardo Turcatti, Benjamin Rappaz, Fabien Kuttler, Bokyoung Kim, Pierre Marquet
We use quantitative phase digital holographic microscopy (QP-DHM) to image and quantify the beating movement of cardiomyocytes, derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. The development of an analysis algo ...
AMER CHEMICAL SOC2020MATHICSE Technical Report : Explicit stabilized multirate method for stiff stochastic differential equations
Assyr Abdulle, Giacomo Rosilho De Souza
Stabilized explicit methods are particularly ecient for large systems of sti stochastic dif- ferential equations (SDEs) due to their extended stability domain. However, they loose their eciency when a severe stiness is induced by very few fast degrees of f ...
EPFL2020Bioengineering the Human Muscle Stem-Cell Niche for Therapeutic Applications
In spite of decades of research, no feasible method for obtaining sufficient numbers of uncommitted muscle stem cells (MuSCs) for therapy of degenerative muscle diseases exists. One of the most fundamental problems associated with stem cell therapy of musc ...
EPFL2019Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells
Matthias Lütolf, Felix Naef, Yoji Tabata, Eric Paquet, François Réal Rivest, Ambroise Roger Vuaridel, Andrea Manfrin
Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...
NATURE PUBLISHING GROUP2019In vivo modeling of human neuron dynamics and Down syndrome
Harnessing the potential of human stem cells for modeling the physiology and diseases of cortical circuitry requires monitoring cellular dynamics in vivo. We show that human induced pluripotent stem cell (iPSC)-derived cortical neurons transplanted into th ...
AMER ASSOC ADVANCEMENT SCIENCE2018Quantitative analysis of SOX2 and OCT4 expression in pluripotency and differentiation
Embryonic stem (ES) cells have the capacity to give rise to all cell types of the adult organism and can be used as a model to study early cell fate decisions as they closely recapitulate in vivo events. The M and G1 phases have been suggested to be the ke ...
EPFL2018