Influence of DNA Binding Dyes on Bare DNA Structure Studied with Atomic Force Microscopy
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The distributions of organic additives in self-leveling flooring compounds (SLCs) were investigated using laser-scanning microscopy for the selective visualization of the additives, which had been previously stained by a fluorescent dye. The distributions ...
Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...
With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...
In view of developing DNA biochips based on electric detection, well controlled DNA functionalisation of conductive substrate electrode is a challenging process. In this study, the whole process of functionalisation onto home made conductive films with con ...
NOVELTY - The micro-array device has several micro-locations (3) associated with a preset DNA sequence. Several micro-sensors are positioned corresponding to the micro-locations. The micro-sensors output electrical signals indicating the absorption of ultr ...
Univ Alma Mater Studiorum Di Bologna; Ricco B; Guiducci C2006
This paper examines reliable advancements in low-cost DNA- and immuno-chips. Capacitance detection was successfully chosen to develop label-free bio-chips. Probe immobilization was rigorously investigated in order to obtain reliable capacitance measurement ...
The thesis presents the development of a custom made atomic force microscope (AFM) conceived to image biological molecules at low temperatures in ultrahigh vacuum conditions, and its application to DNA single molecule studies. Starting from a first prototy ...
Notch1 regulates binary cell fate determination and is critical for angiogenesis and cardiovascular development. However, the pathophysiological role of Notch1 in the postnatal period is not known. We hypothesize that Notch1 signaling in vascular smooth mu ...
PVA coated and fluorescent dye (Cy3.5) functionalized vinyl alcohol/vinyl amine copolymer coated superparamagnetic iron oxide nanoparticles (SPION) were evaluated for systemic distribution and elimination after intraarticular injection in sheep. Observatio ...
First results are reported regarding the design, fabrication and operation of a DNA biochip based on a semiconductor oxide electrode that employs label-free electrical detection of the DNA hybridization. The same process of DNA functionalisation, including ...