A microfluidic platform for high-throughput multiplexed protein quantitation
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Droplet microfluidics has revolutionized quantitative high-throughput bioassays and screening, especially in the field of single-cell analysis where applications include cell characterization, antibody discovery and directed evolution. However, droplet mic ...
NATURE PORTFOLIO2023
The application of microfluidics in the field of surface-based assays and more specifically, the spatial molecular profiling of tumor tissues has gained a lot of interest, especially with the increased interest in personalized medicine and targeted therapy ...
Biochemical reaction rates in microfluidic systems are known to be limited by the diffusional transport of reagents, leading often to lowered sensitivity and/or longer detection times in immunoassays. Several methods, including electrically powering electr ...
ROYAL SOC CHEMISTRY2023
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Emulsion drops are often employed as picoliter-sized containers to perform screening assays. These assays usually entail the formation of drops encompassing discrete objects such as cells or microparticles and reagents to study interactions between the dif ...
Microfluidic is an ensemble of technologies and methods that permits the handling and analysis of minute volumes of samples in short timescales. Since its introduction it revealed a remarkable potential to unveil novel biological mechanisms. The small volu ...
Extracellular vesicles (EVs) are emerging as promising diagnostic and therapeutic tools for a variety of diseases. The characterization of EVs requires a series of orthogonal techniques that are overall time- and material-consuming. Here, a microfluidic de ...
Microfluidics and microtechnologies are of great interest for biological applications. This interest is linked to the fact that microtechnologies enable the study of single cells at the cellular and sub-cellular level. One of many applications of such sing ...
Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...
EPFL2018
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The present invention concerns a system for immunoprecipitation of a protein-DNA complex or for capture of a protein-DNA complex including: - a microfluidic device including a plurality of antibody units, wherein each antibody unit includes at least one mi ...
2019
The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...
