Silver nanoparticle toxicity and association with the alga Euglena gracilis

Renata Behra, Laetitia Bernard, Xiaomei Li, Kristin Schirmer, Laura Sigg
Royal Soc Chemistry, 2015
Article

Résumé

The impact of silver nanoparticles (AgNPs) on aquatic algae has largely been studied with model species that possess a rigid cell wall. Here, we explored the interactions of AgNPs with Euglena gracilis, a green alga having no cell wall but a pellicle. The toxicity and silver uptake upon 1-2 h of exposure to various concentrations of AgNO3 and AgNPs, having a mean size of 47 nm measured in the exposure medium, were examined. The photosynthetic yield decreased in a concentration-dependent manner and AgNPs were less toxic than AgNO3 based on the total silver added. The cell morphology was significantly altered by AgNPs and AgNO3. The damaging effects of AgNPs on the photosynthesis and morphology were completely prevented by cysteine, suggesting that the toxicity of AgNPs was mediated by dissolved Ag. Indeed, the maximal quantity of cell-associated silver was higher upon exposure to AgNPs compared to that upon AgNO3 exposure, amounting to 5.1 x 10(-4) mol L-cell(-1) and 1.4 x 10(-4) mol L-cell(-1) for AgNPs and AgNO3, respectively. However, the difference was not caused by the cellular uptake of AgNPs, but by the strong sorption of AgNPs onto the pellicle.

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Silver nanoparticle effects on simple stream food webs and ecosystem processes

Carmen Omayra Gil Allué

With the global nanotechnology market growing rapidly, nanomaterials are being increasingly released into aquatic environments, where they can undergo modifications and sedimentation, which will put benthic organisms at risk. Of particular interest is the study of nanomaterial effects on periphyton, a community of auto- and heterotrophic microorganisms embedded in an extracellular polymer matrix that covers submerged surfaces in aquatic ecosystems. Although periphyton assumes important functions in ecosystems, like primary production, little information is available on its sensitivity to nanomaterials and how these might be transferred to higher trophic levels in food webs. In the present thesis, effects of short- and long-term exposures to citrate-coated silver nanoparticles (AgNP, 35 nm) and silver ions (Ag+, dosed as AgNO3) were assessed on periphyton in microcosm studies for periods between 2 hours and 21 days. After exposure, primary production, secondary production, photosynthetic yield, microbial respiration and potential extracellular enzyme activity were measured. Moreover, addition of an aquatic snail, Physa acuta, feeding on periphyton allowed the assessment of effects of AgNP on an aquatic herbivore and trophic transfer efficiency. Contaminated (AgNP and AgNO3) and uncontaminated periphyton was offered to snails in a 5-day experiment to determine silver uptake and the mortality, feeding rate and reproduction of the snails. Our findings show that all endpoints measured in periphyton were affected by both AgNP and AgNO3 during short-term exposure, but AgNO3 was more toxic on a total silver mass basis than AgNP. Concentrations causing a 50% reduction in the different endpoints (EC50) ranged from 17 to 83 µM AgNP and from 2 to 4 µM AgNO3. Exposure of periphyton in the presence of silver ion ligands to complex Ag+ (ca. 1% of total silver in AgNP suspensions) prevented AgNP toxicity to most endpoints, confirming that the observed toxic effects were caused by the bioavailable Ag+. An exception was the extracellular enzyme leucine aminopeptidase, which was directly affected by AgNP. Concentrations of AgNP and AgNO3 that caused low toxicity during short-term exposure also caused effects on periphyton functions upon long-term exposure, with algae and bacteria being differently affected. Periphyton exposed to 10 µM AgNP for 21 days showed a 63% decrease in primary production and a 246% increase in bacterial secondary production per unit of total periphyton biomass. The community composition exposed to AgNP or AgNO3 differed from that of the control and also from each other. Furthermore, AgNP-exposed communities were more tolerant to subsequent AgNP exposure. Trophic transfer of silver from contaminated periphyton to snails occurred efficiently, independently of the silver form. Feeding rate of snails on AgNO3-exposed communities was 50% lower than on controls, and disturbance of digestion was evident upon feeding on both AgNP- and AgNO3-exposed communities. No changes in the mortality or egg production of the snails were observed. However, the hatching success of eggs from snails that fed on contaminated periphyton was reduced by more than 79%, and 28-day old embryos showed malformations. Overall, this study clearly identified damaging effects of AgNP on periphyton, with consequent indirect impacts on a consumer, trophic relationships and stream ecosystem processes.

EPFL2015

Chargement

Nanoparticles have various physicochemical properties attributed by their small dimensions. The global production of nanoparticles lead to a raised concern for their environmental impacts. Algae are of highly ecological importance functioning as primary producers for almost all aquatic life. In the present thesis, interactions of nanoparticles with different fresh water algal strains were examined. First, the toxicity and uptake of citrate-coated silver nanoparticles (AgNP) and AgNO3 were examined in the alga Euglena gracilis, which has no cell wall but a pellicle. Secondly, to examine the role of algal cell wall in determining the particles interactions with algae, four strains were selected, including Euglena gracilis, Haematococcus pluvialis, and Chlamydomonas reinhardtii wild type and a cell wall free mutant. Their interactions with polystyrene nanoparticles (PSNP) of two sizes, 50 nm (PSNP50) and 500 nm (PSNP500), were investigated. Third, interactions of three differently coated AgNP with alkaline phosphatase (AP), an extracellular enzyme used for phosphorus acquisition, were assessed. The selected coatings were citrate (CIT), polyvinylpyrrolidone (PVP) and gelatin (GEL). Exposure to AgNP and AgNO3 for 1-2 hours led to decrease in photosynthetic yield, in a concentration-dependent manner, and changes in cell morphology in E. gracilis. Based on total silver added, AgNP were less toxic than AgNO3. Concentrations causing a 50% reduction in photosynthetic yield were 1.9 µM and 85 for AgNP and AgNO3, respectively. Damaging effects of AgNP were completely prevented by cysteine, suggesting that the toxicity of AgNP was mediated by Ag+ ions. Uptake studies showed that the maximal cell-associated silver was higher in AgNP compared to AgNO3, and the higher silver level was shown to correspond to particles adsorbed to the pellicle. By examining four algal stains, it was found that no strain internalized PSNP, emphasizing the role of algal cell walls as barrier for nanoparticle uptake. Interactions of PSNP with algae were found to be unique for each strain, and depend on particle size. PSNP50 were associated with E. gracilis cells displaying a non-homogenous distribution on pellicle. In H. pluvialis, PSNP50 distributed homogenously around the cells. The wild type and cell wall free mutant of C. reinhardtii cells exposed to PSNP50 were found to clump together packed in the extracellular polymeric substances (EPS). The particles were associated with the EPS. The larger PSNP500 were observed to interact only with the two C. reinhardtii strains. Taken together, these results indicate that the algal cell walls hinder the crossing of nanoparticles. Assessing the sorption of AP to AgNPCIT, AgNPPVP, and AgNPGEL showed that the physiochemical properties of both the particle coatings and the enzyme were determinant for the binding. The enzyme adsorbed to AgNPCIT and AgNPPVP, leading to a 10% and 70% coverage of the particle surface area, respectively. No adsorption was found in the case of AgNPGEL. The three types of AgNP decreased AP activity, however, the inhibitory effects only occurred when the AgNP were added after addition of the substrate to the enzyme, not vice versa. AgNO3 did not affect the AP activity. Thus, the results of this study indicate particle-specific effects due to interactions with the AP-substrate intermediate.

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Interaction of silver nanoparticles with algae and fish cells: a side by side comparison

Renata Behra, Xiaomei Li, Kristin Schirmer, Laura Sigg, Yang Yue

Background: Silver nanoparticles (AgNP) are widely applied and can, upon use, be released into the aquatic environment. This raises concerns about potential impacts of AgNP on aquatic organisms. We here present a side by side comparison of the interaction of AgNP with two contrasting cell types: algal cells, using the algae Euglena gracilis as model, and fish cells, a cell line originating from rainbow trout (Oncorhynchus mykiss) gill (RTgill-W1). The comparison is based on the AgNP behavior in exposure media, toxicity, uptake and interaction with proteins. Results: (1) The composition of exposure media affected AgNP behavior and toxicity to algae and fish cells. (2) The toxicity of AgNP to algae was mediated by dissolved silver while nanoparticle specific effects in addition to dissolved silver contributed to the toxicity of AgNP to fish cells. (3) AgNP did not enter into algal cells; they only adsorbed onto the cell surface. In contrast, AgNP were taken up by fish cells via endocytic pathways. (4) AgNP can bind to both extracellular and intracellular proteins and inhibit enzyme activity. Conclusion: Our results showed that fish cells take up AgNP in contrast to algal cells, where AgNP sorbed onto the cell surface, which indicates that the cell wall of algae is a barrier to particle uptake. This particle behaviour results in different responses to AgNP exposure in algae and fish cells. Yet, proteins from both cell types can be affected by AgNP exposure: for algae, extracellular proteins secreted from cells for, e. g., nutrient acquisition. For fish cells, intracellular and/or membrane-bound proteins, such as the Na+/K+-ATPase, are susceptible to AgNP binding and functional impairment.

Biomed Central Ltd2017

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EPFL2015

Silver nanoparticle effects on simple stream food webs and ecosystem processes

Carmen Omayra Gil Allué

EPFL2015