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A Guided Tour of Selected Image Processing and Analysis Methods for Fluorescence and Electron Microscopy

Publications associées (50)

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Imaging green fluorescent protein-labeled neurons using light and electron microscopy

Graham Knott

The ability to observe axons and dendrites with transmission electron microscopy (EM) after they have been previously imaged live with laser-scanning microscopy is a useful technique to study their synaptic connectivity. This protocol provides a detailed m ...

2013

Single-cell lysis for visual analysis by electron microscopy

Henning Paul-Julius Stahlberg

The stochastic nature of biological systems makes the study of individual cells a necessity in systems biology. Yet, handling and disruption of single cells and the analysis of the relatively low concentrations of their protein components still challenges ...

Elsevier BV2013

Functional and Structural Imaging with Optical Coherence Microscopy

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

Is EM dead?

Graham Knott

Since electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed by ...

Company Of Biologists Ltd2013

Live Intracellular Super-Resolution Imaging Using Site-Specific Stains

Suliana Manley, Lina Carlini

Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological feature ...

American Chemical Society2013

Digital confocal microscope

Demetri Psaltis, Alexandre Goy

We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally p ...

Optical Society of America2012

The potential of wood fibers as reinforcement in cellular biopolymers

Pierre-Etienne Bourban, Matthieu Cuénoud, Razvan Cristian Neagu

Wood fiber-reinforced polylactic acid composite foams have been successfully produced using supercritical carbon dioxide. The addition of fibers had a strong effect on microstructure of the foams. An increase in wood fiber content implied smaller average c ...

SAGE Publications2012

Fast Parametric Snakes For 3D Microscopy

Michaël Unser, Ricard Delgado Gonzalo

We present a new fast active contour for images in 3D microscopy. We introduce a fully parametric design that relies on exponential B-spline bases and allows us to impose a sphere-like topology. The proposed 3D snake can approximate blob-like objects with ...

Ieee2012

Fast Parametric Snakes for 3D Microscopy

Michaël Unser, Ricard Delgado Gonzalo

IEEE2012

Comparison of Deconvolution Software: A User Point of View—Part 2

Daniel Sage, Alessandra Griffa

Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...

EMS2010