Highly efficient and gentle trapping of single cells in large microfluidic arrays for time-lapse experiments
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Since the introduction of the of Micro Total Analysis Systems concept in 1990, important progress in microfluidic analytical systems has been realized. In many cases, microfluidic systems have shown improved analytical performances over classical methods, ...
[...] During this project, the fusion feasibility of chips produced for other applications have been tested and some critical fusion parameters have been defined. Then, with the help of FEM simulations, a new chip design has been proposed to overcome the p ...
G-protein coupled receptors (GPCRs) are the most abundant class of proteins in the cell body. Such receptors are of major interest as potential therapeutic targets. Downscaling and parallelization of bioanalytics opens novel routes to rapidly screen and id ...
This thesis reports on the integration of continuous-flow cell separation method for lab-on-a-chip applications. Cell separation methods are widely used in biology to prepare samples prior to analysis. There is a need for a highly sensitive separation meth ...
Optical trapping allows manipulating very small objects – varying from Angstöms to micron size particles – without physical contact by taking advantage of laser light. This technique, relying on light momentum transfer, allows manipulating biological matte ...
The main aim of this project was to develop novel concepts for miniaturization of bioanalytical techniques for investigating biomolecular interactions. We used optical tweezers to selectively address individual biological objects in microfluidic channels. ...
The dynamics of formation of solute peaks in microfluidic systems are investigated by computer simulation. A finite-element numerical procedure is applied to analyze the diffusion- and flow-controlled concentration dispersion in a 40 μm-high rectangular fl ...
This paper reports on a novel method for on-chip continuous separation of divid- ing and non-dividing cells based on differences in their dielectric properties. By means of two opposite dielectrophoretic force fields at multiple frequencies, the two popula ...
We present a 3-D microfluidic device designed for localized drug delivery to cellular networks. The device features a flow cell comprising a main channel for nutrient delivery as well as multiple channels for drug delivery. This device is one key component ...
Microfluidic devices are increasingly used to perform biological experiments on a single-cell basis. However, long-term stability of cell positions is still an issue. A novel biocompatible method for cell entrapment and release on a microchip is presented. ...