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The ultrafast behavior of the ferrous heme f from the cytochrome b(6)f complex of oxygenic photosynthesis is revealed by means of transient absorption spectroscopy. Benefiting from the use of microfluidic technologies for handling the sample as well as from a complementary frame-by-frame analysis of the heme dynamics, the different relaxation mechanisms from vibrationally excited states are disentangled and monitored via the shifts of the heme a-absorption band. Under 520 nm laser excitation, about 85% of the heme f undergoes pulse-limited photo-oxidation (
Margaux Camille Andréa Molinas
Ardemis Anoush Boghossian, Melania Reggente, Mohammed Mouhib
Majed Chergui, Andre Al Haddad, Lars-Hendrik Mewes, Rebecca Ann Ingle