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Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 nm and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes and SNAP-tag are fluorogenic, cell-permeable and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
Marcos Penedo Garcia, Mohammad Shahidul Alam
Beat Fierz, Pablo Rivera Fuentes, Nora Guidotti, Maxime Nicolas Mivelaz, Adam Miklos Eördögh
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