In vivo mechanical loading combined with cell therapy in a bone tissue engineering scaffold
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Background: orthopaedic surgeons often face clinical situations where improved screw holding power in cancellous bone is needed. Injectable calcium phosphate cements are one option to enhance fixation. Methods: paired screw pullout tests were undertaken in ...
In this work we have evaluated the capacity of bone morphogenetic protein-2 (BMP-2) and fibrin-binding platelet-derived growth factor-BB (PDGF-BB) to support cell growth and induce bone regeneration using two different imaging technologies to improve the u ...
A new laser-Doppler flowmeter at large interoptode spacing, based on single-photon counting (single-photon laser-Doppler flowmetry [SP-LDF]) and allowing assessment of blood flow deep in bone tissue, is proposed and implemented. To exploit the advantages o ...
Tissue engineering scaffolds are the new approach to replace common bone grafts. The first aim of this project was to investigate whether bone progenitor cells used in previous studies can undergo osteogenic differentiation. In osteogenic staining experime ...
Excessive interfacial micromotion endangers the implant primary stability by favoring the formation of a fibrous tissue layer at the bone-implant interface. It was recently proposed that implant micromotion induces fluid flow in the granulation tissue that ...
Stem cell therapies hold tremendous potential for tissue and organ regeneration. Yet, there remains significant need for better ex vivo culture and manipulation methods. On the one hand, many tissue-specific stem cells cannot be propagated without causing ...
Bone resorption is seminal for the physiological remodeling of bone during life. However, this process needs to be strictly controlled; excessive bone resorption results in pathologic bone loss, osteoporosis, and fracture. We describe a control mechanism o ...
The Mesenchymal Stem Cell (MSC) is a self-renewing multipotent progenitor originally found in the bone marrow. It has drawn strong interest from translational research because of the multipotency of MSCs, their immunosuppression, their intrinsic homing and ...
Mechanical stimulus has been identified for a long time as a key player in the adaptation of the musculo-skeletal tissues to their function. Mechanical loading is then an intrinsic variable to be considered when new developments are proposed in bone tissue ...
Bone defects in revision knee arthroplasty (RKA) are often located in load-bearing regions. The goal of this study was to determine if a physiologic load could be used as an in situ osteogenic signal to the scaffolds filling the bone defects. In order to a ...