BigNeuron: Large-Scale 3D Neuron Reconstruction from Optical Microscopy Images
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Recent advances in optical microscopy enable the visualization and quantification of biol. processes within live cells. To a great extent, these imaging techniques remain limited by the phys. properties of the chem. probes that are used as fluorescent tags ...
The 2D-molecular thin film analysis protocol for fully grown mice oocytes is described using an innovative approach. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and optical mi ...
Demands of higher spatial and temporal resolutions in linear and nonlinear imaging keep pushing the limits of optical microscopy. We showed recently that a multiphoton microscope with 200 kHz repetition rate and wide-field illumination has a 2–3 orders of ...
A 2016 competition to find the optimum software for 3D single-molecule localization microscopy will help practitioners choose the best tool for the job and spur further developments in the field. ...
We present a semi-blind, spatially-variant deconvolution technique aimed at optical microscopy that combines a local estimation step of the point spread function (PSF) and deconvolution using a spatially variant, regularized Richardson-Lucy algorithm. To f ...
The aims of this study were to (i) investigate the ability of Icelandic brown algae Fucus vesiculosus extracts to inhibit lipid oxidation in granola bars fortified with fish oil-in-water emulsion; (ii) investigate whether addition of the seaweed extracts a ...
Understanding complex physiological processes demands the integration of diverse insights derived from visual and quantitative analysis of bio-image data, such as microscopy images. This process is currently constrained by disconnects between methods for i ...
The development of super-resolved optical microscopies has revolutionized the way we visualize cell biol. These techniques strongly rely on the use of photochem. active fluorophores that display changes in their photophys. properties upon irradn. with ligh ...
Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do ...
We present an algorithm to spatially register two volumetric datasets related via a rigid-body transform and degraded by an anisotropic point-spread-function (PSF). Registration is necessary, for example, when fusing data in multi-view microscopy. Automati ...