GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System
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The emergence of multiple axes is an essential element in the establishment of the mammalian body plan. This process takes place shortly after implantation of the embryo within the uterus and relies on the activity of gene regulatory networks that coordina ...
The bacterial gene regulatory regions often demonstrate distinctly organized arrays of RNA polymerase binding sites of illdefined function. Previously we observed a module of closely spaced polymerase binding sites upstream of the canonical promoter of the ...
American Society for Biochemistry and Molecular Biology2015
Mammalian transcription occurs stochastically in short bursts interspersed by silent intervals showing a refractory period. However, the underlying processes and consequences on fluctuations in gene products are poorly understood. Here, we use single allel ...
Nature / European Molecular Biology Organization2015
The precise tuning of gene expression levels is essential for optimal performance of transcriptional regulatory networks. Many regulatory components control promoter output, but transcription factor (TF) binding sites are amongst the most prominent. Beside ...
Nucleic acid synthesis is spatially organized in many organisms. In bacteria, however, the spatial distribution of transcription remains obscure, owing largely to the diffraction limit of conventional light microscopy (200-300 nm). Here, we use photoactiva ...
Transcription and translation (TX-TL) can be performed in vitro, outside of cells, allowing the assembly and analysis of genetic networks. This approach to engineering biological networks in a less complex and more controllable environment could one day al ...
In mammals, transcription was observed to occur predominantly in bursts, resulting from short and intense periods of gene transcription interspersed by longer silent periods. In order to elucidate the causes and the consequences of discontinuous transcript ...
