Publication
Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Publications associées (35)
Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sagar Shashidhar Manoli
Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...
2012The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability
Florian Maria Wurm, David Hacker, Lucia Baldi Unser
Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable ...
Wiley-Blackwell2011Lentiviral Vector-Mediated Gene Delivery for the Generation of Chinese Hamster Ovary Cell Lines with Improved Productivity and Stability
Recombinant cell line generation by standard transfection techniques is a time-consuming and labor-intensive process often leading to an unpredictable outcome as transgene integration is a rare, random event. Consequently, the population of cells obtained ...
EPFL2010Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production
The growing demand of biopharmaceutical products is boosting the market for therapeutic recombinant proteins (r-proteins). More than half of the 140 r-proteins that have gained approval for human therapeutic use are manufactured in mammalian cells that mak ...
EPFL2010Transient recombinant protein expression in mammalian cells
Transient gene expression (TGE) is a rapid method for generating recombinant proteins in mammalian cells, but the volumetric productivities for secreted proteins in transiently transfected CHO DG44 cells are typically more than an order of magnitude lower ...
EPFL2009Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology
Florian Maria Wurm, David Hacker, Lucia Baldi Unser
Recombinant protein-based biologics are increasingly relevant in the pharmaceutical industry. Currently, mammalian cell-based bioprocesses are a routine manufacturing procedure for complex proteins such as recombinant antibodies, and the number of new biol ...
Wiley2009Studies on scaffold design for tissue engineering applications
The aim of tissue engineering is to regenerate tissue for the purpose of repairing or replacing diseased or injured tissue. Therefore, cells that are able to proliferate are seeded on scaffolds that provide mechanical stability and direct the three-dimensi ...
EPFL2009DNA delivery by microinjection for the generation of recombinant mammalian cell lines
Florian Maria Wurm, David Hacker, Madiha Derouazi, Sébastien Chenuet
Gene transfer methods for producing recombinant cell lines are often not very efficient. One reason is that the recombinant DNA is delivered into the cell cytoplasm and only a small fraction reaches the nucleus. This chapter describes a method for microinj ...
Humana Press2009Simple and efficient transgenesis with meganuclease constructs in zebrafish.
Andrew Charles Oates, Daniele Soroldoni
In the past, microinjection of plasmid DNA into early embryos represented the state of the art to generate transgenic zebrafish. However, this approach suffers significant drawbacks (mosaic distribution of the injected transgene, late transgene integration ...
2009Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics
The forthcoming arrival on the market of numerous protein therapeutics that require high clinical doses will foster the need for high-producing mammalian cell lines. Furthermore, pressures to reduce the development time for new therapeutics has meant more ...
EPFL2008