Publication
Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Publications associées (35)
Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Martin Bertschinger
Human embryonic kidney 293 (HEK293) cells, a widely used host for large-scale transient expression of recombinant proteins, are transformed with the adenovirus E1A and E1B genes. Because the E1A proteins function as transcriptional activators or repressors ...
2004Correlation between cell cycle and calcium phosphate transient transfection of CHO cells
Calcium phosphate transfection is a widely used method to produce recombinant proteins in mammalian cells. However, the mechanisms involved in plasmid DNA transfer to the nucleus of the transfected cells remain poorly understood and result in great variati ...
EPFL2003Large-scale transient expression in mammalian cells for recombinant protein production
Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L ...
1999Integration and stability of CHO amplicons containing plasmid sequences
Fluorescence in situ hybridization (FISH, 15) and high density, non-selective long term perfusion culture were used to study aspects of genetic stability and productivity in recombinant CHO cells. We analysed the distribution and structure of recombinant a ...
1992Allocation of a transgenic c-myc integration site to mouse chromosome 8B3-C1
A recombinant plasmid containing the mouse c-myc gene was injected into mouse pronuclei. The transgenic line 478 contains about 100 copies of the transgene integrated into one chromosome site. By in situ hybridization, the integration site was localized to ...
1988