Nanopore forming method and uses thereof

Method for making nanopores in thin layers or monolayers of transition metal dichalcogenides comprising the steps of provinding a suspended transistion metal dichalcogenide layer (1) in an electrolyte (2), applying a transmembrane voltage (V) high enough to lead to the breakdown of the layer (1), monitoring the ionic current (Ii) and reducing the transmembrane voltage (V) when the ionic current (Ii) has reached a predefined cut-off value (Ip).