Methods for purging isolated organs and cells from latent human cytomegalovirus
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Human embryonic stem (hES) cells can self-renew in culture and differentiate into cells of all three germ layers. Identification of regulators of the self-renewal versus differentiation decisions in hES cells will facilitate manipulation of these cells. Se ...
The early establishment of a reservoir of latently infected T cells is a sobering obstacle to HIV eradication, in spite of the efficacy of current antiretroviral therapies. That latent proviruses might also hide in multipotent hematopoietic stem cells sugg ...
Although canonical Notch signaling regulates multiple hematopoietic lineage decisions including T cell and marginal zone B cell fate specification, the downstream molecular mediators of Notch function are largely unknown. We showed here that conditional in ...
In our laboratory, we have shown previously that bone fetal cells enhance osteogenesis in polymeric scaffolds. However, little is known about the longevity of these cells in vivo, and hence their function. A successful tissue engineering approach needs und ...
DNA-calcium phosphate coprecipitates have been used for 30 years as an efficient method to introduce genetic material into cells. The method involves simple solutions that can be prepared or purchased by the experimentalist. All the numerous variations of ...
A quantitative algorithm was developed and applied to predict target genes of microRNAs encoded by herpesviruses. Although there is almost no conservation among microRNAs of different herpesvirus subfamilies, a common pattern of regulation emerged. The alg ...
Recent advances in nanotechnology led to the development of various nanostructures especially that of carbon-based nanomaterials (CBNs) for industrial and biomedical uses. As a result, the production as well as the human exposure to these materials will in ...
Stem cell use in bladder tissue engineering is a recently addressed area of investigation that has generated excitement as a novel way to restore and regenerate lost or damaged urinary bladder tissue. The remodelling of smooth muscle plays a significant ro ...
Most tissue engineering approaches include the delivery of reparative cells to a damaged host tissue. Generally these cells are sought to be of a stem cell character since they retain a high potential for proliferation and differentiation into diverse phen ...
This unit describes a protocol for the isolation of cells from murine embryonic stem cells with hematopoietic stem cell activity, defined by the ability to reconstitute, long term, multiple lineages of the hematopoietic system of lethally irradiated mice. ...