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Multiple embryo time-lapse imaging of zebrafish development.

Publications associées (34)

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We introduce a microfluidic platform for automated on-chip worm culture, creation of synchronized embryo microarrays, and long-term parallel live imaging of Caenorhabditis elegans embryos. Our device allows studying well-defined embryo populations at unpre ...

2015

Generation of dispersed presomitic mesoderm cell cultures for imaging of the zebrafish segmentation clock in single cells

Andrew Charles Oates, Daniele Soroldoni

Segmentation is a periodic and sequential morphogenetic process in vertebrates. This rhythmic formation of blocks of tissue called somites along the body axis is evidence of a genetic oscillator patterning the developing embryo. In zebrafish, the intracell ...

2014

The zebrafish embryo toxicity test has been proposed as an alternative for the acute fish toxicity test, which is required by various regulations for environmental risk assessment of chemicals. We. investigated the reliability of the embryo test by probing ...

Amer Chemical Soc2012

Axis formation and allocation of pluripotent progenitor cells to the germ layers are governed by the TGF-β-related Nodal precursor and its secreted proprotein convertases (PCs) Furin and Pace4. However, when and where Furin and Pace4 first become active ha ...

Rockefeller University Press2010

Coatings and clusters of carboxylic acids in carbon-containing atmospheric particles from spectromicroscopy and their implications for cloud-nucleating and optical properties

Satoshi Takahama, Shuai Liu

Particle shape and distribution of chemical compounds within individual particles are implied in the parameterizations used in air quality and climate models for radiative transfer, volatility, and mass transfer. In this study we employ Scanning Transmissi ...

2010

ASSET: A robust algorithm for the automated segmentation and standardization of early Caenorhabditis elegans embryos

Pierre Gönczy, Felix Naef, Simon Blanchoud, Yemima Suryani Budirahardja

The early Caenorhabditis elegans embryo is an attractive model to investigate evolutionarily conserved cellular mechanisms. However, there is a paucity of automated methods to gather quantitative information with subcellular precision in this system. We de ...

Wiley-Blackwell2010

ACKGROUND: Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS: The I ...

Oxford University Press2009

Transgenic animals are essential research tools, whether to address basic biological questions or to develop preclinical models of human diseases. Their generation through the injection of naked plasmid DNA into the male pronucleus of a fertilized oocyte h ...

EPFL2009

The tissues of a developing embryo are simultaneously patterned, moved and differentiated according to an exchange of information between their constituent cells. We argue that these complex self-organizing phenomena can only be fully understood with quant ...

2009

Quantitative Morphometrical Characterization of Human Pronuclear Zygotes

Michaël Unser, Philippe Thévenaz

BACKGROUND: Identification of embryos with high implantation potential remains a challenge in in vitro fertilization (IVF). Subjective pronuclear (PN) zygote scoring systems have been developed for that purpose. The aim of this work was to provide a softwa ...

Oxford University Press2008