Publications associées à Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

Suliana Manley, Chen Zhang, Laurent Casini

DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial specie ...

Nature Portfolio2024

C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System

Sebastian Maerkl, Laura Sophie Grasemann

Rolling circle amplification (RCA) is a widely used DNA amplification method that uses circular template DNA as input and produces multimeric, linear single- or double-stranded DNA. Circle-to-circle amplification (C2CA) has further expanded this method by ...

Washington2023

Recycling of Polymerase Chain Reaction (PCR) Kits

Francesco Stellacci, Weina Liu, Yong Zhu

Polymerase chain reaction (PCR) kits have been used as common diagnosing tools during the outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, with daily worldwide usage in the millions. It is well known that at the beginn ...

AMER CHEMICAL SOC2023

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Martinus Gijs, Thomas Lehnert, Lin Sun

Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...

ROYAL SOC CHEMISTRY2022

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Stewart Cole, Charlotte Avanzi, Philippe Busso

Background Diagnosis of leprosy mainly relies on clinical examination due to the inconsistent sensitivity and poor reproducibility of the current laboratory tests. Utilisation of alternative methods to the standard Ziehl Neelsen (ZN), Fite-Faraco (FF) and ...

PUBLIC LIBRARY SCIENCE2018

Spatial confinement induces hairpins in nicked circular DNA

Giovanni Dietler, Aleksandre Japaridze, Enzo Orlandini

In living cells, DNA is highly confined in space with the help of condensing agents, DNA binding proteins and high levels of supercoiling. Due to challenges associated with experimentally studying DNA under confinement, little is known about the impact of ...

Oxford University Press (OUP)2017

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA

Virginija Jovaisaite

Although all Type II restriction endonucleases catalyze phosphodiester bond hydrolysis within or close to their DNA target sites, they form different oligomeric assemblies ranging from monomers, dimers, tetramers to higher order oligomers to generate a dou ...

Oxford University Press (OUP)2017

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

Aleksandra Radenovic, Sebastian Maerkl, Sanjin Marion, Roman Bulushev, Sebastian James Davis, Ekaterina Emilova Petrova

Through the use of optical tweezers we performed controlled translocations of DNA-protein complexes through nanocapillaries. We used RNA polymerase (RNAP) with two binding sites on a 7.2 kbp DNA fragment and a dCas9 protein tailored to have five binding si ...

American Chemical Society2016

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Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System

Recycling of Polymerase Chain Reaction (PCR) Kits

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Exploring on Protein-DNA interactions

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Nanocapillaries combined with optical tweezers as a single molecule technique for studying DNA-protein complexes

Spatial confinement induces hairpins in nicked circular DNA

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

Graph Chatbot

Chattez avec Graph Search

Recycling of Polymerase Chain Reaction (PCR) Kits

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

Exploring on Protein-DNA interactions

Nanocapillaries combined with optical tweezers as a single molecule technique for studying DNA-protein complexes

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA

Spatial confinement induces hairpins in nicked circular DNA