Light availability impacts structure and function of phototrophic stream biofilms across domains and trophic levels

Tom Ian Battin
Blackwell Publishing Ltd, 2018
Article

Résumé

Phototrophic biofilms are ubiquitous in freshwater and marine environments where they are critical for biogeochemical cycling, food webs and in industrial applications. In streams, phototrophic biofilms dominate benthic microbial life and harbour an immense prokaryotic and eukaryotic microbial biodiversity with biotic interactions across domains and trophic levels. Here, we examine how community structure and function of these biofilms respond to varying light availability, as the crucial energy source for phototrophic biofilms. Using metatranscriptomics, we found that under light limitation-dominant phototrophs, including diatoms and cyanobacteria, displayed a remarkable plasticity in their photosynthetic machinery manifested as higher abundance of messenger RNAs (mRNAs) involved in photosynthesis and chloroplast ribosomal RNA. Under higher light availability, bacterial mRNAs involved in phosphorus metabolism, mainly from Betaproteobacteria and Cyanobacteria, increased, likely compensating for nutrient depletion in thick biofilms with high biomass. Consumers, including diverse ciliates, displayed community shifts indicating preferential grazing on algae instead of bacteria under higher light. For the first time, we show that the functional integrity of stream biofilms under variable light availability is maintained by structure–function adaptations on several trophic levels. Our findings shed new light on complex biofilms, or “microbial jungles”, where in analogy to forests, diverse and multitrophic level communities lend stability to ecosystem functioning. This multitrophic level perspective, coupling metatranscriptomics to process measurements, could advance understanding of microbial-driven ecosystems beyond biofilms, including planktonic and soil environments.

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https://infoscience.epfl.ch/record/255570?ln=fr

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Tom Ian Battin
Blackwell Publishing Ltd, 2018
Article

Résumé

Official source

https://infoscience.epfl.ch/record/255570?ln=fr

À propos de ce résultat

Concepts associés (19)

Concepts associés

Chargement

Publications associées (7)

Publications associées

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Genomic and metabolic adaptations of biofilms to ecological windows of opportunity in glacier-fed streams

Tom Ian Battin, Massimo Bourquin, Susheel Bhanu Busi, Vincent Henri De Staercke, Leïla Ezzat, Stylianos Fodelianakis, Tyler Joe Kohler, Grégoire Marie Octave Edouard Michoud, Hannes Markus Peter, Paraskevi Pramateftaki, Martina Andrea Schön, Michail Styllas, Matteo Tolosano

In glacier-fed streams, ecological windows of opportunity allow complex microbial biofilms to develop and transiently form the basis of the food web, thereby controlling key ecosystem processes. Using metagenome-assembled genomes, we unravel strategies that allow biofilms to seize this opportunity in an ecosystem otherwise characterized by harsh environmental conditions. We observe a diverse microbiome spanning the entire tree of life including a rich virome. Various co-existing energy acquisition pathways point to diverse niches and the exploitation of available resources, likely fostering the establishment of complex biofilms during windows of opportunity. The wide occurrence of rhodopsins, besides chlorophyll, highlights the role of solar energy capture in these biofilms while internal carbon and nutrient cycling between photoautotrophs and heterotrophs may help overcome constraints imposed by oligotrophy in these habitats. Mechanisms potentially protecting bacteria against low temperatures and high UV-radiation are also revealed and the selective pressure of this environment is further highlighted by a phylogenomic analysis differentiating important components of the glacier-fed stream microbiome from other ecosystems. Our findings reveal key genomic underpinnings of adaptive traits contributing to the success of complex biofilms to exploit environmental opportunities in glacier-fed streams, which are now rapidly changing owing to global warming.

Springer2022

Chargement

Hydrologic Drivers and Controls of Stream Ecological Processes

Serena Ceola

The temporal variability of streamflows is a key feature structuring and controlling ecological communities and ecosystem processes. The magnitude, frequency and predictability of streamflows, and thus of velocity and near-bed shear stress fields, control ecological structure and function, particularly of benthic organisms. River ecosystem dynamics is indeed deeply connected to streamflow variability, which chiefly relies on rainfall, climate, land use, and geomorphologic properties. Although alterations of streamflow regime due to climate change, habitat fragmentation or other anthropogenic factors are ubiquitous, their ecological implications remain poorly understood. The present Thesis therefore addresses a quantitative analysis of the implications of hydrological fluctuations on river ecosystems positing that they have major ecological importance. From a food-web perspective, the response of benthic biota to hydrologic change is analyzed through experimental and theoretical approaches. Starting from the theoretical characterization of the probability distribution function of streamflows, flow velocities, water depths and near-bed shear stresses at a site, where a comparison between measured and analytical streamflow statistics across several catchments is outlined, a flume experimental campaign has been carried out in order to analyze how flow variability affects biofilm growth and invertebrate grazing activity. Here, two contrasting flow regimes (a constant and a time-varying discharge sequence) and four different light conditions (from 90% to 27% of incoming light radiation), as a key control on biofilm algal productivity and grazer activity, have been performed. Average grazing rates were significantly enhanced under time-varying flow conditions and highest at intermediate light availability. This result suggests that the stochastic flow regime offers increased opportunities for grazing under more favorable shear stress conditions, with implications for stream ecology and trophic carbon transfer in stream food webs. A spatial generalization of the above results, upscaling them to whole river basins, is made possible by employing scaling relationships that are known to characterize the geometry and topology of natural landforms and riverine patterns. The proposed generalization accounts for hydrological characterizations of the driving streamflow variability where the explicit dependence of invertebrate suitability on geomorphic controls (e.g. river depth, bed shear stress and flow velocity) and on hydrologic conditions (e.g. embedded in the probability of streamflow, and the ensuing temporal correlations that define the persistence of the hydrologic signal in time) has been accounted for. Different invertebrate grazing species have been compared. Suitable dynamic models, describing benthic biota dynamics, in particular stream biofilm growth under both ungrazed and grazed conditions (that is, via the absence and presence of invertebrates, respectively), have been explored in order to assess the relevant processes that controlled biofilm-invertebrate temporal pattern. The present Thesis therefore aims to further our understanding of the linkages between hydrology and ecology, possibly leading to a comprehensive theory of hydrologic drivers and controls of biotic processes in stream benthic environments.

EPFL2012

Chargement

Climate change in temperate mountain systems and associated increase in temperature and decrease in precipitation are expected to have strong implications for vegetation productivity, species diversity and carbon turnover in subalpine grasslands. Little is known, however, about the interaction between the effects of climate change and those of local land use management and possible changes in landscape structure. Pasture woodlands in the Swiss Jura Mountains are a traditional landscape, resulting from a long-lived sustainable use of grasslands and woodlands, and as such provide a suite of important ecosystem services to human society. These range from carbon sequestration and biodiversity preservation, to provision of timber and forage for livestock, and last but not least an aesthetic value, much appreciated by tourism. In this thesis various aspects of ecosystem functioning have been studied, investigating the combined effects of experimental climate change and land use on structurally different wooded pastures. An altitudinal gradient method has been used to simulate future climate change conditions, by imposing warmer and drier climate on subalpine turfs transplanted at lower elevation. The resulting gradient in mean annual temperature and precipitation – ranging from cold and wet in the subalpine zone, to warm and dry in the colline zone – has allowed for the detection of tipping points and altered states of ecosystem functioning in response to the treatments. The method employed provided also the possibility for a direct comparison of three land use types: unwooded pastures, sparsely wooded pastures, and densely wooded pastures (the result of pasture management intensity), in their response to climate perturbation. During the four years of experimental work, a series of observations have been made at the plot scale (square metre) in terms of plant performance and biogeochemical cycles, as well as at the landscape scale (hectare) in terms of forage production. A general threshold level for ecosystem resistance to experimental climate change was detected between the moderate IPCC scenario (+2 K mean annual temperature; -20 % annual precipitation) and the intensive IPCC scenario (+4 K mean annual temperature; -40 % annual precipitation). A concomitant gradient in ecosystem response to climate change was observed across the three land use types. The intensively managed unwooded pasture type was consistently more affected by the experimental treatment and rarely exhibited signs of resistance, especially under the intense climate change scenario. A drastic loss of plant species diversity, reduction of herbaceous biomass, impaired litter decomposition and soil microbial metabolic activity have all contributed to the altered state of ecosystem functioning. In contrast, the two extensively managed wooded pasture types showed considerable resistance to climate perturbation in terms of both above and belowground ecosystem processes. The reported inter-annual variation in herbaceous diversity and biomass production within these land use types demonstrated their resilience (recovery) potential too. Using a modelling approach for upscaling these results to the heterogeneous landscape of pasture woodlands in the Swiss Jura Mountains, has proven that extensively used wooded pastures could grant sustainable ecosystem services in terms of forage provision for cattle under climate change. Considering that the two experimental climate change intensities implemented this study are the projected ‘best’ and ‘worst’ case scenarios for the coming decades, the reported resistance of wooded pastures to climate change has to be embraced, and sustainable land use set as a goal in high altitude mountain pastures.

EPFL2013

Chargement

Hydrologic Drivers and Controls of Stream Ecological Processes

Serena Ceola

EPFL2012

EPFL2013