Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection
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The specific labeling of proteins with synthetic probes is a powerful approach to study protein function and protein tags have been widely used for this purpose. A well-established example for a self-labeling protein tag is SNAP-tag. It specifically reacts ...
Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...
The enzymatic hydrolysis of DNA phosphodiester bonds has been widely studied, but the chemical reaction has not yet been observed. Here we follow the generation of a DNA double-strand break (DSB) by the Desulfurococcus mobilis homing endonuclease I-DmoI, t ...
Microfluidic systems have become an attractive platform for diagnostic applications. The technology not only allows drastically decreased assay volumes (down to picoliters), but also accelerates sample processing and readout. In this article we give an ove ...
Although mycobacteria are known to accumulate polyphosphate (PolyP), a linear polymer of orthophosphate, when subjected to stresses, the exact roles of this molecule are not yet clearly understood. We found that deletion of the pstA gene encoding for a tra ...
We present a platform that combines microarrays and microfluidic techniques to measure four protein biomarkers in 1024 serum samples for a total of 4096 assays per device. Detection is based on a surface fluorescence sandwich immunoassay with a limit of de ...
Contamination of drinking water with arsenic is a recurring problem in both industrialized and developing countries. Supplies for large populations can have concentrations much higher than the permissible levels, set by the World Health Organization (WHO) ...
We will demonstrate plasmonic and metamaterial based integrated nano-biosensors and ultrasensitive infrared absorption spectroscopy. These systems by enabling monitoring of molecular-protein interactions in real-time within aqueous solutions can be importa ...
Single molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensiti ...
Understanding protein-protein association and being able to determine the crucial residues responsible for their association (hot-spots) is a key issue with huge practical applications such as rational drug design and protein engineering. A variety of comp ...