Publication
Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates
Publications associées (47)
A Genome-Scale Integration and Analysis of Lactococcus lactis Translation Data
Vassily Hatzimanikatis, Julien Racle
Protein synthesis is a template polymerization process composed by three main steps: initiation, elongation, and termination. During translation, ribosomes are engaged into polysomes whose size is used for the quantitative characterization of translatome. ...
Public Library of Science2013Structural Studies and Protein Engineering of Human O6-Alkylguanine-DNA Alkyltransferase
The specific labeling of proteins with synthetic probes is a powerful approach to study protein function and protein tags have been widely used for this purpose. A well-established example for a self-labeling protein tag is SNAP-tag. It specifically reacts ...
EPFL2012Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability
Ursula Röthlisberger, Kai Johnsson, Florence Pojer, Elizabeth Claire Brunk, Birgit Mollwitz, Simone Schmitt
Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O-6-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to incre ...
2012In Vivo Studies on the Control of Gene Expression and Protein Degradation
Cellular homeostasis is maintained by tightly regulated gene networks, controlled notably at the levels of transcription, mRNA processing, and protein post-translational modification and turnover. This thesis focuses on two of these regulatory steps, namel ...
EPFL2011Gene expression profiling of R6/2 transgenic mice with different CAG repeat lengths reveals genes associated with disease onset and progression in Huntington's disease
R6/2 transgenic mice with expanded CAG repeats (> 300) have a surprisingly prolonged disease progression and longer lifespan than prototypical parent R6/2 mice (carrying 150 CAGs); however, the mechanism of this phenotype amelioration is unknown. We compar ...
Elsevier2011Gamma-Secretase Dependent Gene Expression
Alexandra Marie-Isabelle Karoline Brigitte Magold
The causes of sporadic Alzheimer's Disease (AD), the most common form of dementia at old age, are still unknown. Familial early onset AD (FAD) is mainly caused by mutations of Presenilins (PS), the active subunits of γ-secretase. Processing of its substrat ...
EPFL2011Stochastic noise in translation
Vassily Hatzimanikatis, Julien Racle, Adam Stefaniuk
Translation lies at the heart of every living organism, it is the process synthesizing the proteins, a main component in the cells. Earlier studies have considered protein synthesis as mainly initiation limited, modeling it as a first-order reaction with r ...
2011Stochastic noise in translation
Vassily Hatzimanikatis, Julien Racle, Adam Stefaniuk
Translation lies at the heart of every living organism, it is the process synthesizing the proteins, a main component in the cells. Earlier studies have considered protein synthesis as mainly initiation limited, modeling it as a first-order reaction with r ...
2011Role of proteins in the controlling selenium nanoparticle size
Rizlan Bernier-Latmani, Jan Dobias, Elena Suvorova Buffat
This work investigates the potential for harnessing the association of bacterial proteins to biogenic selenium nanoparticles (SeNPs) to control the size distribution and the morphology of the resultant SeNPs. We conducted a proteomic study and compared pro ...
Institute of Physics2011Rat bladder muscle regeneration induced by local delivery of an engineered insulin-like growth factor 1 in fibrin gels
Currently, both congenital abnormalities and developmental problems of the bladder in children, and other dysfunctions in adults, require reconstructive surgery. Such correction involves transplant action of native tissues (such as gastrointestinal segment ...
EPFL2010