Publication
Integrated microfluidic tools for the characterization of protein/DNA interactions in vitro and in vivo
Publications associées (149)
Statistical learning quantifies transposable element-mediated cis-regulation
Didier Trono, Evaristo Jose Planet Letschert, Julien Léonard Duc, Alexandre Coudray, Julien Paul André Pontis, Delphine Yvette L Grun, Cyril David Son-Tuyên Pulver, Shaoline Sheppard
Background: Transposable elements (TEs) have colonized the genomes of most metazoans, and many TE-embedded sequences function as cis-regulatory elements (CREs) for genes involved in a wide range of biological processes from early embryo- genesis to innate ...
2023Deciphering the molecular events leading to the global restoration of the chromatin landscape during mitotic exit
Two fundamental properties of embryonic stem cells (ESCs) are their ability to self-renew and differentiate into all somatic cell types. Maintenance of their identity faces major challenges when transitioning through mitosis, as most DNA-binding proteins a ...
EPFL2023Development of multiplexed single-molecule imaging techniques to study chromatin invasion dynamics of chromatin effector proteins
DNA-binding proteins physically interact with the DNA and directly affect genomic functions. The eukaryotic genome is compacted into chromatin, limiting the DNA access to nuclear factors. In this Ph.D. thesis, I explored the dynamic mechanisms, that allow ...
EPFL2023A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring
Sebastian Maerkl, Nicolas Rémi Adam, Jonathan Selz
PremiseA novel protocol for rapid plant DNA extraction using microneedles is proposed, which supports botanic surveys, taxonomy, and systematics. This protocol can be conducted in the field with limited laboratory skills and equipment. The protocol is vali ...
WILEY2023iSort enables automated complex microfluidic droplet sorting in an effort to democratize technology
Christoph Merten, Jatin Panwar
Fluorescence-activated droplet sorting (FADS) is a widely used microfluidic technique for high-throughput screening. However, it requires highly trained specialists to determine optimal sorting parameters, and this results in a large combinatorial space th ...
CELL PRESS2023DNA methylation sites in early adulthood characterised by pubertal timing and development: a twin study
Background Puberty is a highly heritable and variable trait, with environmental factors having a role in its eventual timing and development. Early and late pubertal onset are both associated with various diseases developing later in life, and epigenetic c ...
London2023C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System
Sebastian Maerkl, Laura Sophie Grasemann
Rolling circle amplification (RCA) is a widely used DNA amplification method that uses circular template DNA as input and produces multimeric, linear single- or double-stranded DNA. Circle-to-circle amplification (C2CA) has further expanded this method by ...
Washington2023KRAB zinc-finger proteins and their transposable element targets: between antagonism and cooperation
Jonas Caspar De Tribolet-Hardy
There are 377 Krüppel-associated box (KRAB) domain-containing zinc finger proteins (KZFPs) in the human genome, making them the largest family of transcription factors. KZFPs are defined by a N-terminal KRAB domain and several zinc-finger domains arranged ...
EPFL2022Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip
Martinus Gijs, Thomas Lehnert, Lin Sun
Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...
ROYAL SOC CHEMISTRY2022Systematic analysis of biomolecular binding reactions using microfluidic systems
Transcription factor binding to a single binding site on DNA and its functional consequence in a promoter context are beginning to be relatively well understood. However, binding to clusters of sites has yet to be characterized in depth, and the functional ...
EPFL2022