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Imaging cellular ultrastructures using expansion microscopy (U-ExM)

Publications associées (95)

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Morphological investigation of polylactide/microfibrillated cellulose composites

Pierre-Etienne Bourban, John Christopher Plummer, Carole Boissard

Optical microscopy and transmission electron microscopy have been used to investigate the morphology of polylactide (PLA)/microfibrillated cellulose (MFC) composites prepared by: compression molding of wet-comingled MFC and PLA latex or powder, twin-screw ...

Springer Berlin Heidelberg2013

Developing luminescent Brownian probes for near-field investigations of the intracellular environment

Flavio Maurizio Mor

Life sciences have a constantly growing need for novel methodological approaches suitable to investigate the intracellular environment with increased temporal and spatial resolutions. Recently, optical near-field probes, such as laser-irradiated pointed me ...

EPFL2013

Imaging green fluorescent protein-labeled neurons using light and electron microscopy

Graham Knott

The ability to observe axons and dendrites with transmission electron microscopy (EM) after they have been previously imaged live with laser-scanning microscopy is a useful technique to study their synaptic connectivity. This protocol provides a detailed m ...

2013

Is EM dead?

Graham Knott

Since electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed by ...

Company Of Biologists Ltd2013

Advanced Image Processing for Biology, and the Open Bio Image Alliance (OBIA)

Michaël Unser, Daniel Sage, Ricard Delgado Gonzalo

The field of biological imaging has evolved considerably during the past decade as a result of recent (r)evolutions in fluorescence labeling and optical microscopy. Bioimage informatics has been identified as a top priority to cope with the ever-increasing ...

EUSIPCO2013

Digital holographic confocal microscope

Demetri Psaltis, Alexandre Goy

We demonstrate experimentally a scanning confocal microscopy technique based on digital holographic recording of the scanned spot. The data collected in this way contains all the information to produce three-dimensional images. Several methods to treat the ...

Spie-Int Soc Optical Engineering2013

Digital confocal microscope

Demetri Psaltis, Alexandre Goy

We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally p ...

Optical Society of America2012

Resolution and Complex Deconvolution in Holographic Microscopy

Yann Jérôme Michel Pascal Cotte

Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...

EPFL2012

Scanning Near-Field Optical Microscopy of Living Cells in Liquid, Elaboration of New SNOM Probes and Detection Methods

Kanat Dukenbayev

The aim of the present PhD thesis is the elaboration of new, home-made Scanning Near-field Optical Microscope (SNOM or NSOM), and the demonstration of its great potential for high-resolution topography and fluorescence investigations of soft and solid samp ...

EPFL2011

Hard x-ray Zernike microscopy reaches 30 nm resolution

Giorgio Margaritondo, Vincent Gajdosik

Since its invention in 1930, Zernike phase contrast has been a pillar in optical microscopy and more recently in x-ray microscopy, in particular for low-absorption-contrast biological specimens. We experimentally demonstrate that hard-x-ray Zernike microsc ...

Optical Society of America2011