Publication
High-content, cell-by-cell assessment of HER2 overexpression and amplification: a tool for intratumoral heterogeneity detection in breast cancer
Publications associées (50)
Characterization of circulating breast cancer cells with tumorigenic and metastatic capacity
Cathrin Brisken, Georgios Sflomos, Laura Battista
Functional studies giving insight into the biology of circulating tumor cells (CTCs) remain scarce due to the low frequency ofCTCs and lack of appropriate models. Here, we describe the characterization of a novelCTC-derived breast cancer cell line, designa ...
WILEY2020Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study
Martinus Gijs, Diego Gabriel Dupouy, Saska Brajkovic, Fabio Aimi
Breast cancer is a highly heterogeneous disease. The efficacy of tailored therapeutic strategies relies on the precise detection of diagnostic biomarkers by immunohistochemistry (IHC). Therefore, considering the increasing incidence of breast cancer cases, ...
2019Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models
Michele De Palma, Alan Nicolas Thierry Guichard, Mario Leonardo Squadrito, Chiara Cianciaruso, Esra Güç, Ioanna Keklikoglou, Antonino Cassarà
Cytotoxic chemotherapy is an effective treatment for invasive breast cancer. However, experimental studies in mice also suggest that chemotherapy has pro-metastatic effects. Primary tumours release extracellular vesicles (EVs), including exosomes, that can ...
2019ALK7 Signaling Manifests a Homeostatic Tissue Barrier That Is Abrogated during Tumorigenesis and Metastasis
Douglas Hanahan, Nadine Fournier, Mélanie Louise Tichet, Mohammad Sadegh Saghafinia, Iacovos Michael
Herein, we report that the TGF beta superfamily receptor ALK7 is a suppressor of tumorigenesis and metastasis, as revealed by functional studies in mouse models of pancreatic neuroendocrine and lumina! breast cancer, complemented by experimental metastasis ...
CELL PRESS2019Gold and silver bio/nano-hybrids-based electrochemical immunosensor for ultrasensitive detection of carcinoembryonic antigen
Sandro Carrara, Sattar Akbari Nakhjavani, Hadi Afsharan
Carcinoembryonic antigen (CEA) is an important oncomarker for the detection of breast cancer. For ultra-sensitive sensing of CEA with great specificity and accuracy, an innovative and reliable electrochemical immunosensor was developed using various nano-h ...
2019Improvement of breast cancer diagnosis through microfluidics
In breast cancer, 15-20% of cases are reported with overexpression of human epidermal growth factor receptor 2 (HER2) that causes rapid cancer progression and poor prognosis. Fortunately, HER2-targeted therapy using specific antibodies such as Trastuzumab ...
EPFL2018Cell-based quantification of biomarkers from an ultra-fast microfluidic immunofluorescent staining: application to breast cancer cell lines
Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen
Immunohistochemistry (IHC) is one of the main techniques currently used in the clinics for biomarker characterization. It consists in colorimetric labeling with specific antibodies followed by microscopy analysis. The results are then used for diagnosis an ...
SPIE-INT SOC OPTICAL ENGINEERING2018Intraductal patient-derived xenografts of estrogen receptor α-positive breast cancer recapitulate the histopathological spectrum and metastatic potential of human lesions
Cathrin Brisken, Georgios Sflomos, Ayyakkannu Ayyanan, Laura Battista, Maryse Fiche, Valentina Scabia, Patrik Aouad, Valérian Charles Robert Dormoy
Estrogen receptor α-positive (ER-positive) or 'luminal' breast cancers were notoriously difficult to establish as patient-derived xenografts (PDXs). We and others recently demonstrated that the microenvironment is critical for ER-positive tumor cells; when ...
2018Progestins used for hormonal contraception in Switzerland: study of their effects on the breast epithelium
Breast cancer is the most commonly diagnosed cancer and the most common cause of cancer death among women. Hormones have been shown to have a key role in breast development and carcinogenesis. Repeated activation of progesterone receptor (PR) signaling as ...
EPFL2018Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer
Martinus Gijs, Raphaël Etienne Jean Trouillon, Maryse Fiche, Huu Tuan Nguyen, Seiya Matsuoka
Fluorescence in situ hybridization (FISH) is one of the recommended techniques for human epidermal growth factor receptor 2 (HER2) status assessment on cancer tissues. Here we develop microfluidics-assisted FISH (MA-FISH), in which hybridization of the FIS ...
Nature Publishing Group2017