Adaptive damage retention mechanism enables healthier yeast population
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his paper reports on an electrical lysis device with integrated cell counting and evaluation of the lysis efficiency by multiplefrequency impedance measurements. Conventional techniques use chemical reagents to lyse cells, causing unwanted interference wit ...
In Saccharomyces cerevisiae, TBF1, an essential gene, influences telomere function but also has other roles in the global regulation of transcription. We have identified a new member of the tbf1 gene family in the mammalian pathogen Pneumocystis carinii. W ...
A new study of fission yeast cell division has revealed a coupling between cytoplasmic partitioning and the turning-off of cytokinesis signalling that may be mediated by asymmetric protein distribution. ...
The Smg proteins Smg5, Smg6 and Smg7 are involved in nonsense-mediated RNA decay (NMD) in metazoans, but no orthologs have been found in the budding yeast Saccharomyces cerevisiae. Sequence alignments reveal that yeast Ebs1p is similar in structure to the ...
This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicate experimental setups and labeled samples. The use of ...
Gadd45alpha is a nuclear protein encoded by a DNA damage-inducible gene. Through its interactions with other proteins, Gadd45alpha participates in the regulation of DNA repair, cell cycle, cell proliferation, and apoptosis. The NMR structure of human Gadd4 ...
American Society for Biochemistry and Molecular Biology2010
Alginate gel formation on-chip is presented in the first part of this thesis. The technique allows immobilization, and release, of biological cells on-chip. Furthermore, via layer by layer deposition, a locally heterogeneous environment can be generated at ...
New oxathiazinane dioxides have been derived from D- and L-serine and tested for their in vitro cell growth inhibitory activity toward SKBR3 breast cancer cells. (5R)-5-(4-(4'-Bromomethyl)phenyl)benzyloxymethyl-[1,3,4]-oxathiazinane- 3,3-dioxide showed a c ...
In this paper, we present a detailed investigation into the suitability of atomic force microscopy (AFM) cantilevers with integrated deflection sensor and micro-actuator for imaging of soft biological samples in fluid. The Si cantilevers are actuated using ...
In fission yeast, the onset of septation is signalled through the septum initiation network (SIN) signaling pathway. Similarly, in budding yeast the onset of budding is signalled through the mitotic exit network (MEN) pathway. We previously characterized i ...
