Publication
Single-molecule kinetics of pore assembly by the membrane attack complex
Publications associées (43)
Control of nuclear envelope dynamics during acute ER stress by LINC complexes disassembly and selective, asymmetric autophagy of the outer nuclear membrane
The endoplasmic reticulum (ER) extends to the outer (ONM) and the inner (INM) nuclear membrane forming the nuclear envelope (NE) that delimits the nucleoplasm containing the cell genome. Unfolded protein responses (UPRs) and reticulophagy responses increas ...
Philadelphia2024Micro- or nanostructured optical element
Niels Quack, Dorian Giraud Herle
A micro- or nanostructured optical element (1) is proposed comprising: a membrane (3) with an array of holes (5), the membrane (3) comprising a membrane light wave facing surface; an array of pillars (7) sized and shaped such that a respective pillar (7) i ...
2023Water as a contrast agent for imaging interfacial structure and ion transport in giant vesicles
Hydrated lipid bilayer membranes and their asymmetry play a fundamental role in living cells by maintaining and regulating concentration gradients between cells, their environment, and their compartments. They achieve this not only through various channels ...
EPFL2022Characterization of Protein-Membrane Interfaces through a Synergistic Computational-Experimental Approach
The characterization of biological interfaces is widely recognized as one of the main challenges for modern biology. In particular, biological membranes are nowadays known to be an active environment that allows membrane proteins to perform their work and ...
EPFL2020Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin
ABC toxins are pore-forming virulence factors produced by pathogenic bacteria. YenTcA is the pore-forming and membrane binding A subunit of the ABC toxin YenTc, produced by the insect pathogen Yersinia entomophaga. Here we present cryo-EM structures of Yen ...
Springer Science and Business Media LLC2019Characterization of Coenzyme Q Biosynthesis Proteins through Integrative Modeling at the Protein-Membrane Interface
Integral and peripheral membrane proteins account for one-third of the human proteome, and they are estimated to represent the target for over 50% of modern medicinal drugs. Despite their central role in medicine, the complex, heterogeneous and dynamic nat ...
EPFL2019Structure and Activity of the Type VI Secretion System
The type VI secretion system (T6SS) is a multiprotein machine that uses a spring-like mechanism to inject effectors into target cells. The injection apparatus is composed of a baseplate on which is built a contractile tail tube/sheath complex. The inner tu ...
AMER SOC MICROBIOLOGY2019Label-free and charge-sensitive dynamic imaging of lipid membrane hydration on millisecond time scales
Sylvie Roke, Orly Bagunu Tarun
Biological membranes are highly dynamic and complex lipid bilayers, responsible for the fate of living cells. To achieve this function, the hydrating environment is crucial. However, membrane imaging typically neglects water, focusing on the insertion of p ...
2018Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level
Pierre Magistretti, Sylvie Roke, Orly Bagunu Tarun, Pascal Jourdain, Marie Eve Pascale Didier
Neurons communicate through electrochemical signaling within a complex network. These signals are composed of changes in membrane potentials and are traditionally measured with the aid of (toxic) fluorescent labels or invasive electrical probes. Here, we d ...
Nature Publishing Group2018Functional characterization of the virulence determinant ESX-1 from Mycobacterium tuberculosis
Tuberculosis (TB) is a chronic infectious disease that mainly affects the lungs and causes extensive human morbidity and mortality. It results from infection with Mycobacterium tuberculosis, a slow-growing intracellular pathogen that can replicate and surv ...
EPFL2018