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Temporal Super-Resolution Microscopy Using a Hue-Encoded Shutter

Publications associées (55)

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Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

François Thierry M Marelli

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

EPFL2023

Seeing Photons in Color

Edoardo Charbon, Claudio Bruschini, Paul Mos, Mohit Gupta

Megapixel single-photon avalanche diode (SPAD) arrays have been developed recently, opening up the possibility of deploying SPADs as general-purpose passive cameras for photography and computer vision. However, most previous work on SPADs has been limited ...

ASSOC COMPUTING MACHINERY2023

Large-Format Time-Gated SPAD Cameras for Real-Time Phasor-Based FLIM

Arin Can Ülkü

Fluorescence lifetime imaging microscopy (FLIM) is an imaging modality often used to monitor biochemical properties of a cell or a tissue. In addition to conventional fluorescence microscopy features, such as selective labeling and non-invasiveness, FLIM e ...

EPFL2021

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Localization microscopy is a super-resolution imaging technique that relies on the spatial and temporal separation of blinking fluorescent emitters. These blinking events can be individually localized with a precision significantly smaller than the classic ...

2021

How Asynchronous Events Encode Video

Martin Vetterli, Adam James Scholefield, Karen Adam

As event-based sensing gains in popularity, theoretical understanding is needed to harness this technology’s potential. Instead of recording video by capturing frames, event-based cameras have sensors that emit events when their inputs change, thus encodin ...

IEEE2021

Aliasing mitigation in optical microscopy of dynamic biological samples by use of temporally modulated color illumination and a standard RGB camera

Michael Stefan Daniel Liebling, Christian Jaques

Significance: Despite recent developments in microscopy, temporal aliasing can arise when imaging dynamic samples. Modern sampling frameworks, such as generalized sampling, mitigate aliasing but require measurement of temporally overlapping and potentially ...

SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS2020

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Michael Stefan Daniel Liebling, Christian Jaques

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

As of today, the extension of the human visual capabilities to machines remains both a cornerstone and an open challenge in the attempt to develop intelligent systems. On the one hand, the development of more and more sophisticated imaging devices, capable ...

EPFL2020

Generalized temporal sampling with active illumination in optical microscopy

Michael Stefan Daniel Liebling, Christian Jaques

Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overl ...

SPIE2019