Publication
Endogenous fluctuations of OCT 4 and SOX 2 bias pluripotent cell fate decisions
Publications associées (32)
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Separating cells from the body and cultivating them in vitro will alter the function of cells. Therefore, for optimal cell culture in the laboratory, conditions similar to those of their natural growth should be provided. In previous studies, it has been s ...
2021Traceable Impedance-Based Dispensing and Cloning of Living Single Cells
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Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using ...
2020The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells
Precise spatiotemporal regulation of gene expression is essential for development and homeostasis of complex organisms. This is achieved in large part by sequence-specific transcription factors (TF) that bind to genomic regulatory elements to activate or r ...
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Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...
NATURE PUBLISHING GROUP2019Bioengineering the Human Muscle Stem-Cell Niche for Therapeutic Applications
In spite of decades of research, no feasible method for obtaining sufficient numbers of uncommitted muscle stem cells (MuSCs) for therapy of degenerative muscle diseases exists. One of the most fundamental problems associated with stem cell therapy of musc ...
EPFL2019Engineering Dynamic Hydrogels For In Vitro Organoid Culture
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Over the past decade, methods to culture stem cells in three dimensions have opened up a plethora of new opportunities for basic and translational research in the life sciences. In particular, the use of natural extracellular matrix (ECM) surrogates, such ...
EPFL2019Quantitative analysis of SOX2 and OCT4 expression in pluripotency and differentiation
Embryonic stem (ES) cells have the capacity to give rise to all cell types of the adult organism and can be used as a model to study early cell fate decisions as they closely recapitulate in vivo events. The M and G1 phases have been suggested to be the ke ...
EPFL2018A role for mitotic bookmarking of SOX2 in pluripotency and differentiation
Suliana Manley, David Michael Suter, Marion Leleu, Cédric Deluz, Aleksandr Benke, Andrea Callegari, Torgil Elias Friman, Mahé Cécile Raccaud, Daniel Strebinger
Mitotic bookmarking transcription factors remain bound to chromosomes during mitosis and were proposed to regulate phenotypic maintenance of stem and progenitor cells at the mitosis-to-G1 (M-G1) transition. However, mitotic bookmarking remains largely unex ...
Cold Spring Harbor Laboratory2016Stem cell niche engineering through photopatterning and microfluidics
The application of stem cells in drug screening and regenerative therapy has led to important advances in basic biology and biomedicine. Such strategies require high cell numbers and the efficient maturation into faithful functional organ or tissue units. ...
EPFL2016