Publication
Modelling structures in cryo-EM maps
Résumé
Recent advances in structure determination of sub-cellular structures using cryo-electron microscopy and tomography have enabled us to understand their architecture in a more detailed manner and gain insight into their function. The choice of approach to use for atomic model building, fitting, refinement and validation in the 3D map resulting from these experiments depends primarily on the resolution of the map and the prior information on the corresponding model. Here, we survey some of such methods and approaches and highlight their uses in specific recent examples.
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Proximité ontologique
Concepts associés (32)
Transmission electron cryomicroscopy
Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). Cryo-EM is gaining popularity in structural biology. The utility of transmission electron cryomicroscopy stems from the fact that it allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment.
Carte géographique
thumb|right|Esquisse explicative de la plus ancienne carte géographique connue (époque sumérienne, env. 2500 av. J.-C.) vignette|250px|Carte mondiale datant de 1154 réalisée par Al Idrissi pour Roger II de Sicile (ici retournée à ). thumb|right|upright=1.3|Tabula Rogeriana, dessiné par Muhammad al-Idrisi pour Roger II de Sicile (ici retournée à ). Une carte géographique est une représentation d'un espace géographique. Elle met en valeur l'étendue de cet espace, sa localisation relative par rapport aux espaces voisins, ainsi que la localisation des éléments qu'il contient.
Cryomicroscopie électronique
vignette|Un microscope électronique en transmission (2003). La cryomicroscopie électronique (cryo-ME) correspond à une technique particulière de préparation d’échantillons biologiques utilisée en microscopie électronique en transmission. Développée au début des années 1980, cette technique permet de réduire les dommages d’irradiation causés par le faisceau d’électrons. Elle permet également de préserver la morphologie et la structure des échantillons.
Publications associées (43)
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2023MOOCs associés (24)
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Learn about the fundamentals of transmission electron microscopy in materials sciences: you will be able to understand papers where TEM has been used and have the necessary theoretical basis for takin
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Learn about the fundamentals of transmission electron microscopy in materials sciences: you will be able to understand papers where TEM has been used and have the necessary theoretical basis for takin
Synchrotrons and X-Ray Free Electron Lasers (part 1)
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