Publication
Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
Publications associées (41)
Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics
Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...
EPFL2024Deciphering the molecular events leading to the global restoration of the chromatin landscape during mitotic exit
Two fundamental properties of embryonic stem cells (ESCs) are their ability to self-renew and differentiate into all somatic cell types. Maintenance of their identity faces major challenges when transitioning through mitosis, as most DNA-binding proteins a ...
EPFL2023A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
Didier Trono, Gabriela Ecco, Helen Mary Rowe
Mammalian genomes are a battleground for genetic conflict between repetitive elements and KRAB-zinc finger proteins (KZFPs). We asked whether KZFPs can regulate cell fate by using ZFP819, which targets a satellite DNA array, ZP3AR. ZP3AR coats megabase reg ...
2022Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7
Johan Auwerx, Karim Laurent Gariani
Sirtuin 1 (Sirt1) is a NAD(+)-dependent deacetylase capable of countering age-related neurodegeneration, but the basis of Sirt1 neuroprotection remains elusive. Spinocerebellar ataxia type 7 (SCA7) is an inherited CAG-polyglutamine repeat disorder. Transcr ...
2020The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells
Precise spatiotemporal regulation of gene expression is essential for development and homeostasis of complex organisms. This is achieved in large part by sequence-specific transcription factors (TF) that bind to genomic regulatory elements to activate or r ...
EPFL2019DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells
Didier Trono, Julien Léonard Duc, Alexandre Coudray, Alberto De Iaco
After fertilization of the transcriptionally silent oocyte, expression from both parental chromosomes is launched through zygotic genome activation (ZGA), occurring in the mouse at the 2-cell (2C) stage. Among the first elements to be transcribed are the D ...
2019Memory and relatedness of transcriptional activity in mammalian cell lineages
Felix Naef, David Michael Suter, Nicholas Edward Phillips, Aleksandra Mandic, Saeed Omidi Klishami
Phenotypically identical mammalian cells often display considerable variability in transcript levels of individual genes. How transcriptional activity propagates in cell lineages, and how this varies across genes is poorly understood. Here we combine live- ...
2019Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells
Matthias Lütolf, Felix Naef, Yoji Tabata, Eric Paquet, François Réal Rivest, Ambroise Roger Vuaridel, Andrea Manfrin
Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...
NATURE PUBLISHING GROUP2019Protein Engineering Tools to Explore the Function of Protein Post-Translational Modifications for Chromatin and Microtubule Cytoskeletal Biology
Protein post-translational modifications (PTMs) play a crucial role in expanding the protein diversity and are one of the major mechanisms through which cells respond to ever changing environmental cues. The function of two of the most important cellular c ...
EPFL2019Quantitative analysis of SOX2 and OCT4 expression in pluripotency and differentiation
Embryonic stem (ES) cells have the capacity to give rise to all cell types of the adult organism and can be used as a model to study early cell fate decisions as they closely recapitulate in vivo events. The M and G1 phases have been suggested to be the ke ...
EPFL2018